BACKGROUND The rat model of radiation-induced carcinogenesis is widely used to estimate breast cancer risk, but it is unknown whether mammary terminal end buds (TEBs), the hypothesized target of chemical carcinogens, are also targets of radiation-induced carcinogenesis. MATERIALS AND METHODS Female Sprague-Dawley rats were irradiated with X-rays (2 Gy). The morphology, bromodeoxyuridine (BrdU...

Pulse and continuous labelling with tritiated thymidine are used for a quantitative study of cell division rates in regeneration blastemas. Proliferation is initially uniform; later a proximo-distal gradient develops in the mesenchyme, with the highest labelling index at the tip, where practically all cells are shown to be dividing. In the ectoderm there appear to be two growth bands, one close...

Background: Meningiomas are extremely common, slow growing tumours originating from meningeal covering of brain and spinal cord. They mostly encountered in middle or later adult life. Females affected more commonly than males. Grading meningiomas based on histological features has certain limitations predicting exact biological behavior hence ancillary studies like immunohistochemistry can be u...

AIM To compare the Ki-67 labelling index determined using the enhanced peroxidase one step (EPOS) method with that using the standard ABC technique in pituitary tumours. METHODS Adjacent sections were immunostained using the EPOS and ABC techniques with the same Ki-67 antibody and same antigen retrieval method. RESULTS The labelling index measurements with the EPOS Ki-67 antibody were signi...

The factors responsible for collateral arterial growth after major artery occlusion remain obscure, despite their importance for tissue survival. An increase in endothelial cell labelling with tritiated thymidine, as an index of collateral arterial growth, occurs early after renal artery occlusion. Our working premise was that an increase in endothelial cell labelling would occur simultaneously...

Atresia, a degenerative process through which many follicles are removed from the growing pool, involves apoptotic changes in the follicular granulosa cells. To identify histochemical markers of early stages of atresia, an in-vivo rat model was used which allowed the study of atresia of pre-ovulatory follicles in a synchronized and chronological order. By blocking the pre-ovulatory luteinizing ...

The fourth week of postnatal life is a critical point in the development of the hamster Harderian gland. During this week, cells with large lipid vacuoles (type-II cells) appear in the male gland, marking a morphological sex difference that is notorious in adult animals. The origin and fate of type-II cells are controversial. To gain insight into the mechanisms by which type-II cells become a m...

The purpose of this investigation was to study cell proliferation kinetics in human gastric mucosa. Biopsies were taken from the antral and fundic part of the stomach through a fibre-gastroscope and incubated in culture medium containing a DNA-precursor (3-H-thymidine). Autoradiographs were prepared by the dipping technique. The number of labelled cells and the total number of cells in all cros...

Introduction: Cervical cancers are the second most frequent type of female cancer, responsible for about 5% of cancer deaths in females worldwide. Recently, parameters of cell proliferation and cell death have emerged as important diagnostic and prognostic tools. Aims: The aim was to evaluate the role of Apoptotic Index and Ki-67 as proliferation marker in premalignant and malignant lesions of ...