Maillard products arise from condensation reactions between amino acids or proteins with reducing sugars during food processing. As ubiquitous components of human food, these early or advanced glycation products may be subject to intestinal absorption. The present study was performed to investigate the intestinal uptake of Maillard products and to determine whether they are substrates for pepti...

A flow-through analyzer is described for L-lysine concentration determination based on an oxygen electrode and L-lysine-2-monooxygenase immobilized on silica gel. The range of the L-lysine concentration determination is from 5.5 to 55 mM (l-10 g/litre), pH 8-2. The analyzer is characterized by high specificity of the L-lysine determination. The response time is 15-40 s, the total analysis time ...

Penta(L-lysine) and poly(L-lysine) (but not dior tri(-L-lysine)) were found to induce rapid and extensive phospholipid intermixing between unilamellar vesicles consisting largely of phosphatidic acid and phosphatidylethanolamine, without causing significant intermixing of, nor leakage from, the encapsulated aqueous spaces. Neither did the size of such vesicles increase after treatment with lysi...

Pseudomonas putida P2 (ATCC 25571) grown on r,-lysine or DL-pipecolic acid is induced for a high velocity lysine transport system. Double reciprocal plots of L-lysine transport in induced cells are linear. K, and V,,, values are 7.3 X 10V6 M and 130 nmoles per mm per mg for lysinegrown cells and 9.3 X 10e6 M and 42 mnoles per min per mg for cells grown on pipecolate. Lysine transport is optimal...

The yeast Candida albicans is able to utilize L-lysine as the sole nitrogen and carbon source accompanied by intracellular accumulation of alpha-aminoadipate-delta-semialdehyde. A novel yeast amino acid dehydrogenase catalysing the oxidative deamination of the epsilon-group of L-lysine was found in this yeast. The enzyme, L-lysine epsilon-dehydrogenase, is strongly induced in cells grown on L-l...