Flow cytometry (FCM), simple sequence repeats (SSR) and cleaved amplified polymorphic sequence (CAPS) were used to characterize the ploidy level and the nuclear and cytoplasmic compositions of two intergeneric somatic hybrids of Changshou kumquat (Fortunella obovata), leaf parent, and Dancy tangerine (Citrus reticulata), suspension parent. FCM showed that both somatic hybrids were tetraploids. ...

Citrus canker disease caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating biotic stresses affecting the citrus industry. Meiwa kumquat (Fortunella crassifolia) is canker-resistant, while Newhall navel orange (Citrus sinensis Osbeck) is canker-sensitive. To understand the molecular mechanisms underlying the differences in responses to Xcc, transcriptomic profiles of the...

Leaves of 13 interspecific and intergeneric citrus somatic allotetraploid hybrids were analysed and compared with those of their respective parents (lime, lemons, citron, orange, kumquat, poncirus and mandarin) with regards to their sesquiterpenoid contents (μg/g dry weight). The hybrids do not fully retain their parental traits. Sesquiterpenoid production is much reduced (by ~65–90%) in hybrid...

Volatile profiles yielded from gas chromatography-mass spectrometry (GC-MS) analysis provide abundant information not only for metabolism-related research, but also for chemotaxonomy. To study the chemotaxonomy of Mangshanyegan, its volatile profiles of fruit and leaf and those of 29 other genotypes of Citrus, Poncirus, and Fortunella were subjected to phylogenetic analyses. Results showed that...

In this study, the highest oil yield of kumquat peel essential extraction 3.89±0.03% was achieved under supercritical carbon dioxide (SC-CO2) condition including 250 bars, 50°C, fluid rate 20 g/min and 120 min extraction. Limonene comprising 39.25% main component oil. It showed 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) cation radical (ABTS•+) scavenging activity ferric reducing an...

Citrus phylogeny is currently based on genome analysis using molecular markers and sequencing. The 7 pure genetic groups that gave rise to all cultivated citrus underlie the diversity of accessions originating from Asia. However, there are wild forms whose phylogenetic position unknown, such as mountain citron (Citrus halimii B.C. Stone) was discovered in Malaysia early 1970s. We sought elucida...