BACKGROUND NXL104 is a novel-structure beta-lactamase inhibitor with potent activity against both class A and class C enzymes. Among the class A carbapenemases, KPC-type enzymes are now spreading rapidly and KPC-related carbapenemase resistance is an emerging phenomenon of great clinical importance. The activity of NXL104 against KPC beta-lactamases was examined. METHODS Enzymatic activity of...

Klebsiella pneumoniae carbapenemase (KPC) is a carbapenemase increasingly reported worldwide in Enterobacteriaceae. The aim of this study was to analyze the virulence of several KPC-2-producing K. pneumoniae isolates. The studied strains were (i) five KPC-2 clinical strains from different geographical origins, belonging to different ST-types and possessing plasmids of different incompatibility ...

Background and Objective: Biofilm formation plays a crucial role in wound infections and increases the bacteria resistance to treatment. The present study investigated the relationship between the biofilm formation, ESBL, AmpC, and KPC enzymes in Acinetobacter baumannii isolated from the wound specimens. Materials and Methods: Eighty- nine A. baumannii isolates were collected from wound specim...

Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have become more common in the United States and throughout the world. We used pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to examine the molecular epidemiology of KPC-producing K. pneumoniae isolates sent to the Centers for Disease Control and Prevention (CDC) for reference testing from 199...

We developed a novel real-time PCR assay to detect Klebsiella pneumoniae carbapenemases (KPCs) and used this assay to screen clinical isolates of K. pneumoniae and Klebsiella oxytoca for the presence of bla(KPC) genes. The TaqMan real-time PCR assay amplified a 399-bp product from the bla(KPC) gene. The amplicon was designed so that the genes for isoenzymes KPC-1, -2, and -3 could be easily dis...

Carbapenem-resistant Klebsiella strains carrying Klebsiella pneumoniae carbapenemases (KPC) are endemic to New York City and are spreading across the United States and internationally. Recent studies have indicated that the KPC structural gene is located on a 10-kb plasmid-borne element designated Tn4401. Fourteen Klebsiella pneumoniae strains and one Klebsiella oxytoca strain isolated at a New...

In this paper we investigate new sufficient conditions for a digraph to be kernel-perfect (KP) and some structural properties of kernel-perfect critical (KPC) digraphs. In particular, it is proved that the asymmetrical part of any KPC digraph is strongly connected. A new method to construct KPC digraphs is developed. The existence of KP and KPC digraphs with arbitrarily large dichromatic number...

TO THE EDITOR—Klebsiella pneumoniae carbapenemase (KPC)–producing K. pneumoniae (KPC-Kp) has reached a worldwide diffusion, and the associated mortality rate of infected patients is ranging from 45% to 56% [1]. Several risk factors for mortality were identified in patients with KPC-Kp bloodstream infection (KPC-Kp BSI), such as the severity of the underlying disease or the delay in administrati...