A number of reports have appeared in recent years on the mechanism, i.e. the mode of enzyme and substrate interaction, of anaerobic dehydrogenases. For example, the mechanisms of heart muscle lactate dehydrogenase (1)) yeast alcohol dehydrogenase (2), and ribitol dehydrogenase (3) appear to involve a compulsory sequence of enzyme and substrate interaction leading to one or more kinetically impo...

The mechanism of reaction of human erythrocyte hypoxanthine-guanine phosphoribosyltransferase was investigated by initial velocity, product inhibition, and isotope exchange studies. Although initial velocity data are compatible with a mechanism involving binary enzyme-substrate complexes, the product inhibition and isotope exchange studies indicate that the reaction is ordered with the formatio...

Rat skeletal-muscle hexokinase was partially purified by ammonium sulphate fractionation and gel filtration. The mechanism of the skeletal-muscle hexokinase was studied kinetically by initial-velocity analysis and product inhibition. Glucose 6-phosphate was a non-competitive inhibitor of glucose and ATP. ADP was a non-competitive inhibitor of glucose and a competitive inhibitor of ATP. The data...

Polyaniline-fly ash (PANI-FA) composites were prepared by oxidative polymerization of aniline with fly ash in presence of ammonium persulphate (APS). The PANI-FA composites were prepared with different concentrations of fly ash to aniline ratio. The composites, so prepared, were characterized by UV-vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (S...