Confocal or multi-photon laser scanning microscopes are convenient tools to perform FRAP diffusion measurements. Despite its popularity, accurate FRAP remains often challenging since current methods are either limited to relatively large bleach regions or can be complicated for non-specialists. In order to bring reliable quantitative FRAP measurements to the broad community of laser scanning mi...

objectives: curcumin has anti-inflammatory and antioxidative properties. the objective of this study was to investigate the therapeutic effects of curcumin on functional disturbances, oxidative stress, and leukocyte infiltration induced by renal ischemia/reperfusion (i/r). materials and methods: animals were randomly divided into 9 groups. the groups with 24-h reperfusion consisted of sham-24h,...

OBJECTIVES Gentamicin is used for the treatment of Gram-negative bacterial infections. However, gentamicin administration is limited because of nephrotoxicity. The aim of the present study was to evaluate the protective effect of crocin against gentamicin-induced nephrotoxicity in rats. MATERIALS AND METHODS Thirty two male Wistar rats received gentamicin (100 mg/kg, IP), with or without croc...

Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool towards analysing diffusion processes within living biological cells. A model is presented that aims to provide a rigorous theoretical framework from which binding information of proteins from FRAP data can be extracted. A single binding reaction is considered and a set of mathematical equations is introduced...

background: gentamicin (gm) is one the aminoglycoside antibiotics which isroutinelyused to treatinfections gram-negative, either alone or insynergistic withbeta-lactamantibioticsused. however, frequent useleads toserious side effectssuch asrenal toxicity, ototoxicity. coenzyme q10 has antioxidant, anti-inflammatory and vasodilatory properties. according to these properties of coenzyme q10 and t...

enhanced oxidative stress in diabetes mellitus may contribute to the pathogenesis of diaetic complications. in this article, the stress oxidative generation has been studied in experimental diabetes by ferric reducing ability of plasma assay, a sensitive and simple method, and by other oxidative damage markers. the ferric reducing ability of plasma values as a total antioxidant capacity were si...

A procedure for a systematic evaluation of fluorescence recovery after photo-bleaching (FRAP) data is presented that allows one to determine distributions of diffusion coefficients. The method provides a straightforward and calibration-free way to quantify multiple diffusion processes when FRAP is measured as a function of time and space by means of confocal laser scanning microscopy. It is ver...

The analogous techniques of photoactivation of fluorescence (PAF) and fluorescence recovery after photobleaching (FRAP) have been applied previously to the study of actin dynamics in living cells. Traditionally, separate experiments estimate the mobility of actin monomer or the lifetime of actin filaments. A mathematical description of the dynamics of the actin cytoskeleton, however, predicts t...

OBJECTIVE Boerhaavia elegans L. (Nyctaginaceae) is a medicinal plant used for the treatment of kidney disorders, urinary tract disorders and blood purification in Baluch tribe. The aim of present study is to evaluate the antioxidant property of B. elegans species for the first time. MATERIALS AND METHODS Different parts (leaf, stem and fruit) of the plant were extracted by using various solve...