要 旨 単 純 ヘ ル ペ ス ウ イ ル ス1型(HSV-1)お よ び2型(HSV-2)の 遺 伝 子 を 同時 に検 出 す るmultiplex real-time PCR法 を 開発 し た. HSV-1お よびHSV-2のgpD領 域 を標 的 と し,プ ラ イ マ ー お よ びTaqMan蛍 光 プ ロ ー ブ を 独 自 に 設 計 し た.検 出感 度 は15copies/tubeで,HSV-1お よ びHSV-2と も15か ら1.5×106 copies/tubeの 範 囲 で 定 量 が 可 能 で あ っ た.real-time PCR法 はPCR法 よ り も103倍 感 度 が 高 く,他 の ヘ ルペ ス属(CMV, EBV, HHV6, HHV-7お よ びVZV) DNAは 検 出 され なか っ た. 以 上 の結 果 か ら,今 回 開 発...

SUMMARY Diagnostic residual and partial residual plots are proposed to identify nonlinearity in covariates for generalized linear models. It is shown that these plots are complement each other in the detection of nonlinearity. In addition, a transformed additive model which allows the resulting residual and partial residual plots to be obtained through the standard least squares approach is pro...

â  a geographical dimension in the poetry of de’bil al- khozaee â  â  abdul rasoul ghaffari * â  â abstractâ in this article, we attempt to study de'bil al-khozaee's poetry. we find him a person who travelled a lot around the west and the east of islamic world. in his journey, he acquired knowledge, belles-letters, morals and also wealth. what is important for us in this research is...