Previous studies have shown that pretreatment with sub-lethal kanamycin to E. coli B23 cells provided protection against subsequent exposure to lethal amounts of the same antibiotic. It has also been shown that exposure to sub-inhibitory levels of certain antibiotic can induce cross resistance by promoting the development of low-level resistance to other functional and structural unrelated anti...

A small group of institutionalized Down’s syndrome patients were treated with topical kanamycin sulphate paste, once or twice a day for 3-5 days, every fifth week over a 52-week-period. The teeth were not brushed during this 52-week-period. The wet weight of the supragingival plaque in the kanamycin group was significantly reduced when compared to the supragingival plaque formed in the group re...

Kanamycin production by Streptomyces kanamyceticus ATCC 12853 requires magnesium sulfate and potassium phosphate at concentrations of 0.4 and 1.0 g per liter, respectively. The optimal concentrations of Fe and Zn for production of kanamycin are 0.25 and 0.575 mug/ml, respectively, whereas Mo at 0.04 mug/ml allows maximal cellular growth and antibiotic synthesis. Mn and Ca are without any effect...

The emergence of multidrug-resistant (MDR) tuberculosis (TB) highlights the urgent need to understand the mechanisms of resistance to the drugs used to treat this disease. The aminoglycosides kanamycin and amikacin are important bactericidal drugs used to treat MDR TB, and resistance to one or both of these drugs is a defining characteristic of extensively drug-resistant TB. We identified mutat...

Metabolite-enabled killing of antibiotic-resistant pathogens by antibiotics is an attractive strategy to manage antibiotic resistance. Our previous study demonstrated that alanine or/and glucose increased the killing efficacy of kanamycin on antibiotic-resistant bacteria, whose action is through up-regulating TCA cycle, increasing proton motive force and enhancing antibiotic uptake. Despite the...

Aminoglycoside antibiotics, such as kanamycin, have ototoxic side effects, which often result in degeneration of cochlear and vestibular hair cells in the inner ear. Cytotoxic effects of aminoglycosides, however, do not appear immediately after cellular uptake of aminoglycosides. In order to understand the mechanisms responsible for the delayed emergence of aminoglycoside ototoxicity, changes i...