نتایج جستجو برای: k atpase
تعداد نتایج: 402068 فیلتر نتایج به سال:
We previously have demonstrated that the colonic P-ATPase alpha subunit cDNA encodes an H,K-ATPase when expressed in Xenopus laevis oocytes. Besides its high level of amino acid homology (75%) with the Na,K-ATPase, the colonic H,K-ATPase also shares a common pharmacological profile with Na,K-ATPase, because both are ouabain-sensitive and Sch 28080-insensitive. These features raise the possibili...
Ouabain inhibited 86RbCl uptake by 80% in rabbit gastric superficial epithelial cells (SEC), revealing the presence of a functional Na+,K+-ATPase [(Na+ + K+)-transporting ATPase] pump. Intact SEC were used to study the ouabain-sensitive Na+,K+-ATPase and K+-pNPPase (K+-stimulated p-nitrophenyl phosphatase) activities before and after lysis. Intact SEC showed no Na+,K+-ATPase and insignificant M...
PURPOSE It has been established that Na,K-ATPase activity is higher in lens epithelium than fibers. However, others have suggested the Na,K-ATPase enzyme may be inactive or absent in the central 10% of the epithelium. Studies were conducted to measure and compare Na,K-ATPase specific activity and to examine Na,K-ATPase protein expression in the anterior and equatorial regions of porcine lens ep...
این تحقیق بمنظور تعیین مراحل پار و اسمولت و تعیین اندازهای از بچه ماهی آزاد دریای خزر (salmo trutta caspius kessler,1877) جهت تحمل آب دریای خزر انجام گرفت. در این تحقیق از 4 گروه وزنی 5، 10، 15 و 20 گرمی در سه تیمار شوری آب دریا (5/11-11 در هزار)، آب ساحل (7 در هزار) و آب شیرین و با 3 تکرار استفاده شد. متوسط فشار اسمزی بچه ماهیان 5، 10، 15 و 20 گرمی در زمان صفر بترتیب معادل 1/7±301، 6/14±7/334...
سابقه و هدف: رده سلولی 4t1 کارسینومای پستان موش، یک مدل آزمایشگاهی است که به صورت گسترده در مطالعه بیولوژی تومور ها کاربرد دارد. این سلول ها به شدت تومورزا و مهاجم بوده و دارای قابلیت بالای متاستاز در اندام های دیگر هستند. این مطالعه به منظور بررسی ساختار و مکان یابی آنزیمna+ , k+ –atpase ، تومور های ایجاد شده توسط رده سلولی 4t1 در موش balb/c nu به روش بافت شناسی و ایمونوهیستوشیمی انجام شد. موا...
Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the pumping function of Na/K-ATPase, and instead depend on interaction between Na/K-ATPase and Cav1 ...
Changes in Na(+)/K(+)-ATPase activity and abundance associated with environmental salinity were investigated in the gills and rectal gland of the Atlantic stingray Dasyatis sabina. Using a ouabain-specific ATPase assay and western blotting, we found that stingrays from fresh water had the highest activity and highest relative abundance of Na(+)/K(+)-ATPase in the gills. Using immunohistochemist...
Cerebral ischemia-reperfusion injury (CIRI) is one of the main diseases leading to death and disability. Studying role mechanism drugs in mitigating CIRI great significance. In present study, effect formononetin from Trifolium pratense L. on oxidative stress, energy metabolism impairment inflammatory response after cerebral mice were investigated. Formononetin was extracted purified. The treate...
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