Analysis of the extent and distribution of genetic diversity in crop plants is essential for optimizing sampling and breeding strategies. Several methods were identified for the existence of diversity in maize for the aim of perfect selection based on great diversity among maize genotypes. In this study, Inter-simple sequence repeat (ISSR) markers were used to assess genetic diversity in a sele...

The genetic relationship among some naturally growing Vicia taxa in Turkey (V. sativa subsp. sativa, subsp.nigra var.nigra, subspincisavar.cordata, V. craccasubsp.cracca, subspgerardii, subspatroviolacea, subspstenophylla, V. hybrida, V. peregrinaandV. palaestina) was assessed by helping ISSR markers. During PCR assays, although 17 primers were tested, only three primers produced informatics am...

Necrophagous fly is the most common insect evidence collected during a death investigation. We analyzed the DNA polymorphism among the five forensically important fly species, namely, Lucilia sericata, Aldrichina grahami, Chrysomya megacephala, Parasarcophaga crassipalpis and Musca domestica using inter simple sequence repeat (ISSR) method. Nine ISSR primers selected from 18 primers could ampli...

The variability level of the ISSR (inter-simple sequences repeat) primer (GACA)4 was examined in the three Lepidoptera families Pyralidae, Sphingidae and Pieridae. Our study shows that the tetra-repeat (GACA)n is evidently present in sufficient numbers in these butterflies to provide informative DNA fingerprints. The variability is mostly rather high, but within a comparable range to other ISSR...

Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...

In the present study, genetic diversity among 16 collections of Stevia rebaudiana Bertoni was assessed to compare the efficiency of two-marker systems over one marker using RAPD and ISSR markers. How well these two marker systems discriminated the Stevia collections was compared. In fact, all the Stevia accessions included in the present study have been growing under different altitudes, rangin...

Evaluation of relationships is of prime importance in any breeding studies. In current research, genetic diversity among different rapeseed genotypes from several geographical origins including Canada, Australia, Germany, France, Denmark, Netherlands, Sweden, Iran, and Somegenotypesof unknown origin, were evaluated by RAPD and ISSR markers. All of the generated bands using 12 RAPDs and 15 ISSRs...

Apart from being fed to silkworms in sericulture, the ecologically important Mulberry plant has been used for traditional medicine in Asian countries as well as in manufacturing wine, food, and beverages. Germplasm analysis among Mulberry cultivars originating from South Korea is crucial in the plant breeding program for cultivar development. Hence, the genetic deviations and relations among 8 ...

Haematococcus pluvialis (Flotow) is a unicellular green alga, which is considered to be the best astaxanthin-producing organism. Molecular markers are suitable tools for the purpose of finding out genetic variations in organisms; however there have been no studies conducted on ISSR or RAPD molecular markers for this organism. The DNA of 10 different strains of H. pluvialis (four strains from Ir...

Brazil is considered one of the domestication centers of cassava (Manihot esculenta), containing a large part of the biological diversity and traditional knowledge of the species. The aim of the present study was to evaluate the genetic diversity of cassava landraces grown by farmers in the north of Mato Grosso State, Brazil, using inter simple sequence repeat (ISSR) molecular markers. The stud...