The isozyme patterns of six different enzymes and the polypeptide profiles of soluble proteins have been examined in muscle biopsy specimens from 74 patients with a wide variety of neuromuscular disorders. About half of the samples showed unusual features in at least one, and often several, of the enzymes and proteins tested. The extent of the biochemical abnormalities was roughly proportional ...

In the CNS, there are multiple isozymes of the sodium and potassium ion-stimulated adenosine triphosphatase (Na,K-ATPase) that have differences in affinity for Na+, ATP, and cardiac glycosides. Three forms of the catalytic subunit (designated alpha 1, alpha 2, and alpha 3) are known to be derived from different genes, but little is known of the cellular distributions of the proteins or their ph...

The regulatory subunits of both isozymes of cAMP-dependent protein kinase bind 2 mol of cAMP/mol of monomer. cAMP dissociation studies indicate similar cAMP binding behavior for each isozyme. Each has two different intrachain cAMP binding components present in approximately equal amounts and the rate of cAMP dissociation is 5- to 10-fold slower from one site (Site 1) than from the other (Site 2...

Twenty-two continuous cell lines derived from normal and neoplastic urothelium, maintained under identical culture conditions, were characterized in terms of isozyme phenotype, tumorigenicity, and xenograft morphology following xenotransplantation to nude mice, cytological appearance, in vitro growth rate, labelling index, and colony-forming efficiency, in parallel with separate studies of in v...

Three isozymes of catechol 1,2-dioxygenase (pyrocatechase) from Pseudomonas at-villa C-l were separated using DEAE-Toyopearl chromatography. The specific activities of each isozyme were similar to one another. The molecular weights of isozymes 1, 2, and 3 were estimated to be approximately 67,000,64,000, and 59,000, respectively, from gel filtration. On sodium dodecyl sulfate-polyacrylamide gel...

drought is one of the most important factors limiting crop yields around the world. drought stress in plants, the change (increase or decrease) in the production of plant proteins. this research was carried out using bread wheat genotypes. for evaluation of leaf protein pattern in wheat, 10 genotypes were assayed with 3 replications under irrigated (non-stress) and rain-fed (stress) conditions....

Drought is one of the most important factors limiting crop yields around the world. Drought stress in plants, the change (increase or decrease) in the production of plant proteins. This research was carried out using bread wheat genotypes. For evaluation of leaf protein pattern in wheat, 10 genotypes were assayed with 3 replications under irrigated (non-stress) and rain-fed (stress) conditions....

Protein banding pattern of eight snakeheads – Channa species viz., Channa striatus, Channa marulius, Channa punctatus, Channa diplogramme, Channa bleheri, Channa gachua, Channa stewartii and Channa aurantimaculata collected from different regions of India were used to study the phylogenetic relationship among them. The banding pattern from muscle protein indicated a unique profile for each spec...

The isozymes of malic dehydrogenase (MDH) of maize have been separated by starch gel zone electrophoresis. The MDH isozyme pattern was the same in different organs and developmental stages. The subcellular distribution of the different isozymes was established by means of differential centrifugation. The MDH isozymes were found to be differentially distributed in the cytoplasm, the mitochondria...

Changes in the lactate dehydrogenase (LDH) isozyme pattern of primary culture of neurons treated with botulinum C3 enzyme were examined in order to elucidate the functional changes accompanying the morphological change that follows ADP-ribosylation of Rho protein. Primary neurons were prepared from the cerebrum of ICR mouse embryos on day 15. Neurons were cultured in MEM with 10% fetal calf ser...