G-quadruplexes are higher-order nucleic acids structures formed by G-rich sequences that are stabilized by tetrads of hydrogen-bonded guanine bases. Recently, there has been growing interest in the study of G-quadruplexes because of their possible involvement in many biological processes. Isothermal titration calorimetry (ITC) has been proven to be a useful tool to study the energetic aspects o...

Isothermal titration calorimetry (ITC) provides highly complementary data to high-resolution structural detail. An overview of the methodology of the technique is provided. Ultimately, the correlation of the thermodynamic parameters determined by ITC with structural perturbation observed on going from the free to the bound state should be possible at an atomic level. Currently, thermodynamic da...

0022-2836/$ see front matter q 2004 E Abbreviations used: AcWL5, acet isothermal titration calorimetry; DS scanning calorimetry; TM, transmem palmitoyl-oleoyl-phosphocholine. E-mail address of the correspond [email protected] The hexapeptide acetyl-Trp-Leu5 (AcWL5) has the remarkable ability to assemble reversibly and spontaneously into b-sheets on lipid membranes as a result of monomer partit...

The hammerhead ribozyme undergoes a well-defined two-stage conformational folding process, induced by the binding of magnesium ions. In this study, we have used isothermal titration calorimetry to analyze the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme. Binding to the natural sequence ribozyme is strongly exothermic and can be analyzed in terms of seque...

The hydration of the lipid 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) and of the cationic detergent dodecyltrimethylammonium bromide (DTAB) has been studied by means of isothermal titration calorimetry (ITC), gravimetry, and infrared (IR) spectroscopy. During the experiments films of the amphiphiles are perfused by an inert gas of variable relative humidity. The measurement of adsorption he...

Caries-associated biofilms induce loss of calcium from tooth surfaces in the presence of dietary carbohydrates. Exopolysaccharides (EPS) provide a matrix scaffold and an abundance of primary binding sites within biofilms. The role of EPS in binding calcium in cariogenic biofilms is only partially understood. Thus, the aim of the present study is to investigate the relationship between the calci...

We studied by native ESI-MS the binding of various DNA-polymerase-derived peptides onto DNA-polymerase processivity rings from Escherichia coli, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. These homodimeric rings present two equivalent specific binding sites, which leads to successive formation during a titration experiment of singly- and doubly occupied rings. By using the ESI-MS f...

human serum albumin (hsa) is the most abundant protein in the blood plasma. drug binding to hsa is crucial to study the absorption, distribution, metabolism, efficiency and bioavailability of drug molecules. in this study, isothermal titration calorimetry and molecular dynamics simulation of hsa and its complex with indometacin (im) were performed to investigate thermodynamics parameters and th...

The dairy industry uses enzymes to make cheese, alter product flavor, and eliminate lactose. activities of these have been measured in clear buffered solutions, but because the limitations spectrophotometric methods, enzyme not opaque or colored products where they are used. Isothermal titration calorimetry (ITC) can be used determine reaction kinetics solutions by measuring heat rate (thermal ...