To what extent does glutamine turnover keep pace with oxidative metabolism in the rat heart? To address this question, the following groups of substrates were presented to the isolated, working rat heart: 1) glucose (5 mM), insulin (40 microU/ml), and [2-13C]acetate (5 mM; high workload, n = 5); 2) pyruvate (2.5 mM) and [2-13C]acetate (5 mM; normal workload, n = 5); or 3) propionate (1 mM) and ...

imatinib is an orally administered tyrosine kinase inhibitor which inhibits the bcr-abl protein-tyrosine kinase with high selectivity. imatinib is rapidly absorbed from the gut, after oral intake and has an almost absolute bioavailability of 98%. the metabolism of imatinib is mediated by the cytochrome p450 (cyp) isoenzymes in the liver and gut wall. cgp74588 is a major active metabolite of ima...

A simple method has been developed for continuous monitoring of metabolic activity of an isolated, perfused rat heart by O2/CO2 respirometer. Since respirometer provides vital data on oxygen consumption and carbon dioxide production of a preserved organ on a continuous basis over a long period of time, it will be possible to use this method to monitor viability of not only isolated heart but al...

Objective: To assess whether the protective effect of ischaemic preconditioning (IPC) on endothelial function in coronary arteries of the rat involves prostaglandins. Methods: Isolated rat hearts perfused under constant flow conditions were exposed to 30 min of partial ischaemia (flow-rate 1 ml /min) followed by 20 min of reperfusion, after which coronaries were precontracted with U-46619 0.1 m...

background chronic hypoxia causes apoptosis of cardiac myocytes, however, energy production by anaerobic glycolysis protects myocardium against hypoxia injuries. aldolase a is a well-characterised key enzyme of the glycolysis pathway. ghrelin, a 28-amino-acid peptide, synthesizes in the stomach and has protective roles in cardiovascular systems and also affects metabolic pathways. objectives th...

The present study was focused on possible effects of highly selective, high affinity sigma receptor ligand BD737 in rat isolated hearts perfused according to Langendorff. Electrogram recorded by touch-less method and left ventricular pressure acquired by balloon method were followed during acute exposure of isolated rat heart to nanomolar concentrations of the drug. Ventricular ectopic beats an...

previous studies demonstrated that cse induces oxidative stress and its consequences on isolated mitochondria obtained from lung, heart and brain which may provide insight into the role of cse in human health and disease. the present study was carried out to further characterize and compare toxic effect of cse extract on isolated mitochondria obtained from either a directly contacting tissue (i...

background & objectives: cardiovascular effects of herbal medicine were shown in numerous studies. falcaria vulgaris or gazayag has traditionally been used in kermanshah as an herbal medicine and its beneficial effect on peptic ulcer and skin wound has been proved in previous studies. there is some evidence about its cardiac effect. in addition, special chemical substance called butylidenephtha...

BACKGROUND/AIM There is no information on the dose-response relationship of curcumin on the hemodynamic variables of the heart at the organ level in isolated perfused rat hearts. We aimed to investigate the effects and mechanisms of curcumin on the hemodynamic variables of isolated perfused rat hearts. MATERIALS AND METHODS Rats were randomly divided into 9 groups. The isolated rat heart was ...

objective(s) this study aimed to examine whether acetyl-l-carnitine (alc) was able to reduce cardiac arrhythmias and infarct size in the ischemic-reperfused isolated rat heart. materials and methods the isolated hearts were mounted on a langendorff apparatus then perfused by a modified krebs-henseleit solution during 30 min regional ischemia and 120 min reperfusion (control) or by enriched kreb...