The increasing need for alternative fuels to replace fossil has made bioethanol a promising option. Although numerous sources of sugar generation and agricultural wastes can be converted into ethanol, Avocado Seeds (AS) are particularly attractive as raw materials due their abundance, high carbohydrate content, lack interactions with the food chain. Therefore, this study investigated potential ...

In response to DNA damage or the inhibition of normal DNA replication in Escherichia coli, a set of some 20 unlinked operons is induced through the RecA-mediated cleavage of the LexA repressor. We examined the effect of this SOS response on the transposition of Tn5 and determined that the frequency of transposition is reduced 5- to 10-fold in cells that constitutively express SOS functions, e.g...

This communication reports an analysis of Tn5yIS50 target site selection by using an extensive collection of Tn5 and IS50 insertions in two relatively small regions of DNA (less than 1 kb each). For both regions data were collected resulting from in vitro and in vivo transposition events. Since the data sets are consistent and transposase was the only protein present in vitro, this demonstrates...

Tn5 transposase (Tnp) binds to Tn5 and IS50 end inverted repeats, the outside end (OE) and the inside end (IE), to initiate transposition. We report the isolation of four Tnp mutants (YH41, TP47, EK54 and EV54) that increase the OE-mediated transposition frequency and enhance the binding affinity of Tnp for OE DNA. In addition, two of the Tnp mutants (TP47 and EK54) appear to be change-of-speci...

Transposition of Tn5 and IS50 requires the specific binding of transposase (Tnp) to the end inverted repeats, the outside end (OE) and the inside end (IE). OE and IE have 12 identical base-pairs and seven non-identical base-pairs. Previously we described the isolation of a Tnp mutant, EK54, that shows an altered preference for OE versus IE compared to wild-type (wt) Tnp. EK54 enhances OE recogn...

Granite deposits have a strategic importance due to the energy developments in Türkiye and its stability plays an important role environmental safety. Therefore, it is of major for engineering practice investigate thermal damage mechanisms case high temperature exposure effects. This study discusses results tests performed physico-mechanical microstructural properties granite after temperatures...

Purpose: To assess the degree of conversion (DC) dual-curing composite cements when cured through ceramic-veneered zirconia disks. Methods: Portions mixed cement, either G-CEM LinkForce (GC), Panavia V5 (Kuraray Noritake) or ResiCEM (Shofu), were placed on ATR crystal a Fourier Transform Infrared Spectroscope (FTIR; iS50, Thermo Scientific) and squeezed to 100-µm film thickness using microscopy...

TnphoA construct was derived from the transposon Tn5, which contained a kanamycin resistance gene flanked by two IS50 elements. This construct contains a version of the alkaline phosphatase gene with its signal sequence and promoter deleted, which will result in a blue colony phenotype on X-phos (5-bromo-4-chloro-3-indolyl phosphate toluidine salt) containing media when secreted into the peripl...

To identify host genes that might influence nod (nodulation) gene expression in Rhizobium leguminosarum, a nodC-phoA reporter plasmid (carrying nodD) was introduced into a chemically mutagenized population of a R. leguminosarum strain lacking a symbiotic plasmid. The transconjugants were screened for expression of alkaline phosphatase (PhoA) on plates containing hesperetin, an inducer of nod ge...