The Na/Ca exchanger is associated with 160, 120 and 70 kDa polypeptides whose nature is poorly understood. We have purified and characterized the Na/Ca exchanger from bovine cardiac sarcolemmal vesicles (SLVs) by using ion-exchange and affinity chromatographies. The Na/Ca exchanger-enriched fraction was reconstituted into asolectin liposomes [lipid to protein ratio 10:1 (w/w)] that showed Na/Ca...

transport by the human Na ϩ /Ca 2ϩ exchanger expressed in Sf 9 cells. Am. block of the Na ϩ /Ca 2ϩ exchanger was examined in Sf 9 cells expressing the human heart Na ϩ /Ca 2ϩ exchanger (NCX1-NACA1). As predicted from the reported actions of Ni 2ϩ , its application reduced extracellular Na ϩ-dependent changes in intracellular Ca 2ϩ concentration (measured by fluo 3 fluores-cence changes). Howeve...

Thin ion-selective membrane films deposited on solid electrode substrate are useful tools to study ion transfer processes. This is because the experimental conditions may be chosen such that diffusion processes within and contacting aqueous solution not rate limiting. In an ideal case, therefore, equilibrium considerations used describe resulting voltammograms. For example, electrochemical oxid...

Cl(-) and K(+) activities in the follicular lumen and the intraluminal potential of the same lumen were measured simultaneously with specific liquid ion-exchanger and 3 M KCl microelectrodes, respectively, in turtle thyroid glands. The Cl(-) equilibrium potential between the thyroid interstitium and the lumen calculated from the measured Cl(-) activities was higher than the directly measured in...

Poly-o-anisidine Sn(IV) arsenophosphate is a newly synthesized nanocomposite material and has been characterized on the basis of its chemical composition, ion exchange capacity, TGA-DTA, FTIR, X-RAY, SEM, and TEM studies. On the basis of distribution studies, the exchanger was found to be highly selective for lead that is an environmental pollutant. For the detection of lead in water a heteroge...