The role of structural signals in intercompartmental transport has been addressed by the isolation of yeast invertase (SUC2) mutations that cause intracellular accumulation of active enzyme. Two mutations that delay transport of core-glycosylated invertase, but not acid phosphatase, have been mapped in the 5' coding region of SUC2. Both mutations reduce specifically the transport of invertase t...

Some strains of Candida utilis produce exceptionally large amounts of extracellular and total invertase. Strain Y-900 of C. utilis produces high yields whether the carbon source is sucrose, glucose, maltose, or xylose and still higher yields with lactic acid, glycerol, and ethyl alcohol. Approximately 20 to 30% of the total invertase of C. utilis is extracellular. Strains of Saccharomyces cerev...

The growth of baker's yeast Saccharomyces cerevisiae in medium containing sucrose requires a high level of extracellular invertase enzyme activity. However, the expression of invertase is under the strict control of glucose repression in S. cerevisiae. In addition, invertase enzyme activity is also affected by physiological stress conditions that baker's yeast is exposed to during the various s...

A novel technique was adopted using wood waste as a new carrier for adsorption of invertase. The new method improved activity, pH and thermal stability of the immobilized invertase from Saccharomyces cerevisiae and was more resistant to washing out by concentrated salt solution (6M NaCl). This result encourages using sawdust as matrix for purification of invertase or may be for other enzymes. O...

1. Some 16 amino acids (not containing SH or S-S groups) did not affect the reactivation of yeast invertase inactivated by acid. 2. Cysteine, reduced glutathione, homocysteine, thiophenol, and thioglycollic acid accelerated the reactivation of yeast invertase. 3. Cystine, oxidized glutathione, and homocystine inhibited the reactivation of yeast invertase. 4. The compounds mentioned in 2 and 3 d...

Invertase (EC.3.2.1.26) catalyzes the hydrolysis of sucrose to an equimolar mixture of D-glucose and D-fructose which is of interest for various industrial applications. In this research, Saccharomyces cerevisiae invertase gene (SUC2) was optimized based on Pichia pastoris codon preference. The synthetic gene was introduced into the methylotrophic yeast Pichia pastoris under the control of the ...

The soluble invertase activity in etiolated Avena seedlings was highest at the apex of the coleoptile and much lower in the primary leaf, mesocotyl, and root. The activity in all parts of the seedling consisted of two invertases (I and II) which were separated by chromatography on diethylaminoethylcellulose. Both enzymes appeared to be acid invertases, but they differed in molecular size, pH op...

This communication describes a method for adsorbing the invertase (EC.3.2.1.26) on DOWEX® anion exchange resin. Among the types of DOWEX® resins studied (1x8:50-400; 1x4:50-400 and 1x2:100400), 1X4-200 was the most suitable, because it adsorbed the invertase molecules completely and the complex 1X4-200/invertase retained 100% of the catalytic activity. Moreover, no leakage of enzyme from the su...

It has been observed by Michaelis and Pechstein (1) and others, that a-methyl glucoside manifests a marked retarding influence on the hydrolysis of sucrose by invertase. The curves given in Fig. 1 represent the hydrolysis of a 10 per cent sucrose solution by invertase in the presence and absence of the glucoside. Nelson and Hitchcock (2) found that the hydrolysis of 10 per cent sucrose solution...

BACKGROUND: Plant invertases play important roles in sucrose metabolism. Cell wall invertase has been reported to participate in phloem loading and unloading. Soluble invertases are involved in hexose level regulation in mature tissues and in utilisation of stored sucrose within vacuoles. Invertase inhibitory proteins have been described as one of the possible components for invertase activity ...