Character and rapidity of protein diffusion in intracellular fluids are key determinants of the dynamics and steady state of a plethora of biochemical reactions. So far, an anomalous diffusion in cytoplasmic fluids with viscoelastic and even glassy characteristics has been reported in a variety of organisms on several length scales and timescales. Here, we show that the contiguous fluid of form...

Aquaporins represent a primordial group of transmembrane solvent channels that have been documented throughout the living biota. This facet alone emphasizes the positive selection pressure for proteins associated with intracellular fluid homeostasis. Amongst extant Eukaryota the highest gene copy number can be found in plants and teleosts, a feature that reflects the genomic duplication history...

When cells of a marine pseudomonad washed free of medium components with 0.05 m MgSO(4) were suspended in solutions containing 200-mm concentrations of various salts, there was an immediate increase in optical density (OD), followed by a slow decrease. The decrease following the initial increase, but not the increase itself, could be prevented by omitting K(+) from or by adding metabolic inhibi...

The objective of this experiment was to establish sex-related differences in the parameters bioimpedance spectroscopy Wistar rat’s bodies experimental metabolic syndrome (endocrine-salt model).
 Sexual dependencies measurements intact animals have been determined for first time: males higher amount total fluid, but lower one fat. intra/extracellular fluid balance is characterized with rati...

The relative volumes of major body fluids of freshwater and marine sharks are remarkably similar in spite of the differences in external medium and in osmotic pressure of body fluids. The small differences detected are in agreement with differences reported in comparisons of freshwater and marine teleosts: a slightly higher total water content and a smiller ratio of extracellular to intracellul...

American Society of Brewing Chemists (1944). Method8 of Analy8i8, Malt-6, 4th ed. Wisconsin: A.S.B.C. Blom, J. & Rosted, C. 0. (1947). Acta chem. Scand. 1, 32. Claus, W. D. (1947). Cereal Chem. 24, 59. Freeman, G. G. & Hopkins, R. H. (1936). Biochem. J. 30, 446. Graesser, F. R. & Dax, P. J. (1946). Wallerstein Lab. Comm. 9, 43. Hopkins, R. H., Murray, R. H. & Lockwood, A. R. (1946). Biochem. J....

BACKGROUND Markers of ventilator associated pneumonia are of interest for confirming the diagnosis and for guiding the initial management of this frequent complication of mechanical ventilation. The detection of intracellular organisms in the polymorphonuclear leucocytes (PMNLs) and/or macrophages of bronchoalveolar lavage (BAL) fluid has been suggested as a specific test for the early indicati...

We investigated acid-base permeability properties of electrically resistive monolayers of alveolar epithelial cells (AEC) grown in primary culture. AEC monolayers were grown on tissue culture-treated polycarbonate filters. Filters were mounted in a partitioned cuvette containing two fluid compartments (apical and basolateral) separated by the adherent monolayer, cells were loaded with the pH-se...

We investigated the mechanisms by which serine proteases alter lung fluid clearance in rat lungs and vectorial ion transport in airway and alveolar epithelial cells. Inhibition of endogenous protease activity by intratracheal instillation of soybean trypsin inhibitor (SBTI) or alpha(1)-antitrypsin decreased amiloride-sensitive lung fluid clearance across rat fluid-filled lungs; instillation of ...

Swystun, Veronica, Lan Chen, Phillip Factor, Brian Siroky, P. Darwin Bell, and Sadis Matalon. Apical trypsin increases ion transport and resistance by a phospholipase C-dependent rise of Ca . Am J Physiol Lung Cell Mol Physiol 288: L820–L830, 2005. First published December 30, 2004; doi:10.1152/ajplung.00396.2004.— We investigated the mechanisms by which serine proteases alter lung fluid cleara...