Chitosan (CS) and chondroitin sulfate (CHS) are natural polymers with demonstrated applicability in drug delivery, while nanoparticles are one of the most explored carriers for transmucosal delivery of biopharmaceuticals. In this work we have prepared CS/CHS nanoparticles and associated for the first time the therapeutic protein insulin. Fluorescein isothiocyanate bovine serum albumin (FITC-BSA...

The article notes the latest presented work on electrochemical detection of insulin and presents a critical view research development its non-enzymatic sensors. It monitors effect expe­rimental conditions oxidation signal considers catalytic effects nanoparticles or nanocomposites deposited surfaces sensor.

OBJECTIVE To determine whether and by what mechanism systemic delivery of curcumin-containing liposomes improves insulin resistance in the leptin deficient (ob/ob) mouse model of insulin resistance. RESEARCH DESIGN AND METHODS Insulin resistant ob/ob mice with steatosis were injected intraperitoneally with liposome nanoparticles, entrapping the nuclear factor-κB (NF-κB) inhibitor curcumin (cu...

The aim of this work was to produce and characterize cetyl palmitate-based solid lipid nanoparticles (SLN) containing insulin, and to evaluate the potential of these colloidal carriers for oral administration. SLN were prepared by a modified solvent emulsification-evaporation method based on a w/o/w double emulsion. The particle size, zeta potential and association efficiency of unloaded and in...

PLGA nanoparticles are useful to protect and deliver proteins in a localized or targeted manner, with a long-term systemic delivery pattern intended to last for a period of time, depending on polymer bioerosion and biodegradability. However, the principal concern regarding these carriers is the hydrolytic instability of polymer in aqueous suspension. Freeze-drying is a commonly used method to s...

Diabetes mellitus represents one of the most widespread diseases in civilization nowadays. Since costs for treating and diagnosing diabetes represent several billions dollars per year, a cheap, fast, simple sensor diagnosis is needed. Electrochemical insulin sensors can be considered as novel approach diagnosis. In this study, carbon electrode with electrodeposited NiO nanoparticles was selecte...

Two major hurdles need to be surmounted for cell therapy for diabetes: (i) allo-immune rejection of grafted pancreatic islets, or stem/precursor cell-derived insulin-secreting cells; and (ii) continuing auto-immunity against the diabetogenic endogenous target antigen. Nanotherapeutics offer a novel approach to overcome these problems and here we ask if creation of "stealth" islets encapsulated ...

Cytotoxicity is a severe problem for cadmium sulfide nanoparticles (CSNPs) in biological systems. In this study, mercaptoacetic acid-coated CSNPs, typical semiconductor Q-dots, were synthesized in aqueous medium by the arrested precipitation method. Then, amino-terminated polyethylene glycol (PEG) was conjugated to the surface of CSNPs (PCSNPs) in order to introduce amino groups to the surface....

Proteins and peptides play an important role in seizure prevention and neuroprotection and development of CNS. Acute increase in peripheral insulin level leads to higher CSF insulin, while chronic peripheral hyper-insulinemia down regulates insulin receptors in BBB, impairing insulin transport in the brain. Thus, increased insulin level in the circulatory pathway can affect the brain’s normal a...