background: rotaviruses cause diarrhea in infants and young children worldwide. rotavirus outer capsid protein, vp7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. many efforts have been done to produce recombinant vp7 that maintain native characteristics. we used baculovirus expression system to produce rotavirus vp7 protein and to s...

background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...

Members of the family Baculoviridae are insect-specific dsDNA viruses that have been used for biological control of insect pests in agriculture and forestry, as well as in research and pharmaceutical protein expression in insect cells and larvae. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the type species of the family Baculoviridae. During infection of AcMNPV in permissiv...

Influenza vaccines have long been manufactured in embryonated chicken eggs. This method has some problems such as a production period (about 6 months) and use of large amounts infectious pathogens. Recently, the recombinant subunit using baculovirus–insect cell system extensively investigated. In this system, viral immunodominant components can be produced more rapidly larger scale than convent...