We have compared the recF genes from Escherichia coli K-12, Salmonella typhimurium, Pseudomonas putida, and Bacillus subtilis at the DNA and amino acid sequence levels. To do this we determined the complete nucleotide sequence of the recF gene from Salmonella typhimurium and we completed the nucleotide sequence of recF gene from Pseudomonas putida begun by Fujita et al. (1). We found that the R...

The objective of this study is to investigate the efficiency of calcium carbonate bioprecipitation by Lysinibacillus sphaericus, Bacillus subtilis and Pseudomonas putida, obtained from the Coleção de Culturas do Instituto Nacional de Controle de Qualidade em Saúde (INCQS), as a first step in determining their potential to protect building materials against water uptake. Two culture media were s...

Autoclaving of crude oil is often used to evaluate the hydrocarbon-degrading abilities of bacteria. This may be potentially useful for bioaugmentation and microbial enhanced oil recovery (MEOR). However, it is not entirely clear if "endogenous" bacteria (e.g., spores) in/on crude oil survive the autoclaving process, or influence subsequent evaluation of the hydrocarbon-degradation abilities of ...

The use of plant growth-promoting rhizobacteria (PGPR) is a sustainable way for efficient absorption and utilization nutrients by crop species to enhance growth yield persistence against biotic abiotic stresses. However, the PGPRs in drought tolerant crops such as foxtail millet limited because low adaptability moisture conditions. In this study, acclimatised conditions, were isolated from rhiz...

Alternative microbial hosts have been engineered as biocatalysts for butanol biosynthesis. The butanol synthetic pathway of Clostridium acetobutylicum was first re-constructed in Escherichia coli to establish a baseline for comparison to other hosts. Whereas polycistronic expression of the pathway genes resulted in the production of 34 mg/L butanol, individual expression of pathway genes elevat...

Rising carbon dioxide (CO2) concentrations in atmosphere have a significant impact on plant growth and metabolism. Many promoting microbes play an important role maintaining vitality by facilitating nutrient translocation under adverse conditions. This study was carried out to investigate the effects of elevated CO2 parameters (height seedlings, increment height, collar diameter number leaves) ...

We report the draft genome sequence of Pseudomonas putida JLR11, a facultative anaerobic bacterium that has been studied in detail for its capacity to use the explosive 2,4,6-trinitrotoluene (TNT) as a nitrogen source. The sequence confirms the mechanisms used by this versatile strain to reduce and assimilate nitrogen from TNT.

Pseudomonas putida strain ASAD was isolated from compost because of its ability to utilize aspirin (acetylsalicylic acid) as a carbon and energy source. We report the draft genome sequence of strain ASAD, with an estimated length of 6.9 Mb. Study of this isolate will provide insight into the aspirin biodegradation pathway.

Plant Growth Promoting Rhizobacteria Pseudomonas and Bacillus are well known biofertilizer due to their ability for solubilizing inorganic phosphate producing phytohormones in soil. However, potential decreased diseases incidence caused by Pratylenchus coffeae has been documented. The objective of this laboratory experiment was formulate solid contained diminuta subtilis. experiments consisted ...