A procedure was developed for 4-h identification of bacteria from blood culture. From a turbid blood culture bottle a 10- to 15-ml aliquot was drawn off and centrifuged. The pellet was utilized to inoculate a series of enzymatic and physiologic reactions. Three hundred eight positive blood cultures were tested including 222 strains of Enterobacteriaceae, 40 strains of facultative grampositive c...

Saprophytic growth of Phytophthora cinnamomi indicated that the pathogenicity was not enhanced by addition of Nor Ca-sources to the basal growth medium. Mycelium obtained from the N and Ca-enriched sources and used to inoculate blue lupin seedlings indicated that the pathogenicity of the fungus was reduced by the addition of either N or Ca to the fungus cultures. The detached root technique was...

Rhinocerebral mucormycosis is an acute fulminant opportunistic fungal infection usually seen in diabetic or immunocompromised patients. The fungi that cause mucormycosis inoculate the nasal mucosa and may spread to the paranasal sinuses, orbit, and brain. Our patient initially presented with mild ethmoid sinusitis. At that time, brain MRI and contrast-enhanced MR angiography were grossly normal...

A method was developed for susceptibility testing with spherule-endospore-phase Coccidioides immitis by using a microtiter format. Isolated endospores were used to inoculate wells containing modified Converse medium with various concentrations of azole or nikkomycin antifungal substances which then were sealed with an acetate film. The plate was incubated at 37 degrees C with shaking for 96 h, ...

In the simplest version of this activity, students inoculate a yeast suspension into glucose solutions of several concentrations. The glucose disappears, and the amount of CO2 produced is directly proportional to the initial amount of sugar in solution. This suggests a simple model of yeast fermentation. To challenge this model, students can perform parallel experiments in which the glucose con...

A 39-year-old man with no prior history of underlying arthritis developed osteomyelitis and septic arthritis in his hand following a cat bite. This case illustrates the virulence of Pasteurella multocida infections associated with animal bites, particularly those of cats, whose teeth can inoculate bone directly. The onset of cellulitis caused by P. multocida infections is often rapid, and the d...

A nuclear polyhedrosis virus isolated from infected Bombyx mori, BmNPV, was used to inoculate silkworms to determine salivary gland cell susceptibility. The salivary gland was removed from infected silkworms at different times post-inoculation and examined by light microscopy. The salivary gland cells did not exhibit any signs of BmNPV infection; however, fat body and tracheal cells, used as po...

was able to obtain large volumes of high potency material by batch cultivation in tanks, but did not report any yields. was sterilized at 118 to 122 C for 1 hr, cooled rapidly to 36 to 39 C, and inoculated immediately. Seed cultures of Clostridium tetani (Massachusetts strain) were maintained at -70 C in 1.0-ml amounts in glass ampoules. One or four of these ampoules were thawed and inoculated ...

8. How to examine for plague bacteriologically. 9. How to prepare a vaccine. 10. How to inoculate antitoxic serums. 11. How to treat a case of snake-bite. 12. How to examine for spirochceta pallida. 13. How to use Salvarsan. 14. How to identify mosquitoes. 15. How to test for albumen in urine. 16. How to test for blood in urine. 17. How to examine feces for amoebae. 18. How to inject quinine fo...

Three combined triple antigen vaccines were used to inoculate infants receiving primary immunization at 3 to 6 months of age. Laboratory potency and toxicity tests and clinical evaluation again showed that the mouse weight gain test is able to predict which vaccines will give reactions in children. The addition of aluminium hydroxide to the vaccine both increased potency and reduced the tendenc...