Antibodies that bind to human platelet membrane glycoproteins IIb and IIIa were used to develop methods for analyzing platelet membrane components by flow cytometry. Platelets were tentatively identified by their low-intensity light scatter profiles in whole blood or platelet-rich plasma preparations. Identification of this cell population as platelets was verified by using platelet-specific an...

A variety of drugs that are generally thought to modify biological membrane action may affect platelet function [ 11. Phospholipase A? is a membrane-bound enzyme, and plays a key role in the release of arachidonic acid from platelet membrane phospholipids [ 21. The arachidonic acid thus released is converted by platelet cyclo-oxygenase and lipoxygenase pathways to prostaglandin endoperoxides, t...

Platelet membrane glycoproteins IIb and IIIa were isolated and purified from human platelet membranes using lentil lectin affinity chromatography and electrophoretic elution from sodium dodecyl sulfate-polyacrylamide gels. Two-dimensional immunoelectrophoresis of a mixture of the purified proteins against monospecific antisera showed antigenic uniqueness of the separate polypeptides. Compute...

Platelets have previously been shown to contain a membrane skeleton that is composed of actin filaments, actin-binding protein, and three membrane glycoproteins (GP), GP Ib, GP Ia, and a minor glycoprotein of Mr = 250,000. The present study was designed to determine how the membrane glycoproteins were linked to actin filaments. Unstimulated platelets were lysed with Triton X-100, and the membra...

The polysulfone-based membrane has been used in hemodialysis (HD) and it is continuously developed to maintain its sustainability on the subject of biocompatibility. During the polysulfone-based membrane development, several parameters should be considered to improve the membrane performances, such as excellent biocompatibility, appropriate ultrafiltration rate, and effective clearance of the t...

The recently shown transmissibility of variant Creutzfeldt-Jakob disease (vCJD) by blood transfusion emphasises the need for better understanding of the cellular prion protein (PrPc) in blood. A substantial amount of cell-associated PrPc in blood resides in platelets. Platelet activation leads to up-regulation of PrPc on the platelet surface and its release on exosomes and microparticles. The s...

Arachidonate-mediated release of 14C serotonin and thromboxane B2 (TXB2) is significantly enhanced in the presence of complement. Only purified complement components C5, C6, C7, C8, and C9 are required for this reactivity. No known activating mechanism of the classical or alternative pathway is required, nor is C3. In the absence of exogenously added complement, platelet membrane-bound compleme...

The role of extravascular coagulation in vitreous membrane formation was investigated in rabbits and owl monkeys. Eyes injected with platelet-poor plasma did not develop vitreous membranes. In contrast, the injection of platelet-rich plasma and gel-filtered platelet preparations into the vitreous cavity resulted in immediate membrane formation. These vitreous membranes were only slowly and inco...