www.spec t roscopyon l i ne . com T he concept of step-scan mid-infrared inter ferometr y dates back to the 1960s when the first optoelectronically controlled step-scan interferometer was reported (1). In contrast to the linear change of the optical path difference (retardation) in a conventional continuous-scan interferometer, the retardation changes stepwise in a step -scan Fourier transform–...

A high-throughput screening system for Citrus lines were established with higher sugar and acid contents using Fourier transform infrared (FT-IR) spectroscopy in combination with multivariate analysis. FT-IR spectra confirmed typical spectral differences between the frequency regions of 950-1100 cm(-1), 1300-1500 cm(-1), and 1500-1700 cm(-1). Principal component analysis (PCA) and subsequent pa...

The application of single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy was investigated for secondary structure analysis of 15 representative proteins in H2O solution. This is the first reported application of single-pass ATR-FT-IR for protein analysis; thus, the method was validated using transmission FT-IR and multipass ATR-FT-IR as referee methods. The s...

The European project FAIR-CT96-5053 concerned the application of the FT-Raman and infrared spectroscopy in food chemistry and quality control. Our research mainly concerned the study of the potential of Raman spectroscopy and the comparison with the results achieved in infrared spectroscopy. The discrimination of virgin olive oil from other edible oils, and the detection and quantification of v...

As near infrared spectra has the characters of multi-variables and strong correlations, to solve the problem, Fourier transform (FT) was used to extract feature variables of shelled chestnuts spectra. FT coefficients and the status of 178 chestnuts were selected as inputs and outputs of the back-propagation neural network (BPNN) classifier to build a recognition model. For comparison, principal...

The FTIR and FT-Raman spectra of 3-methyl-4-nitrophenol (MNP) have been recorded in the regions 4000-400 cm and 3500-50 cm, respectively. Utilizing the observed FTIR and FT-Raman data, a complete vibrational assignment and analysis of the fundamental modes of the compound have been carried out. The optimum molecular geometry, harmonic vibrational frequencies, infrared intensities and Raman scat...

The Fourier Transform Infrared (FT-IR) spectroscopy is by far known to be a useful technique for qualitative and quantitative analysis of asbestos in bulk samples, since all asbestos species exhibit intense absorption peaks in the 4000 400 cm region of the infrared spectrum. In the present work, we compare the accuracy and precision of two analytical procedures (the Linear Calibration Curve Met...

The Fourier transform gas phase (5000-400 cm), solid phase (4000-400 cm) infrared spectra as well as Raman spectra (3500-50 cm) of 1,3-dimethylbarbituric acid have been recorded. Quantum chemical calculations of energies, geometrical structure and vibrational wave numbers of 1,3-dimethylbarbituric acid have been made by density functional method with 6-31G(d,p) as basis set. The best level of t...

Alicyclobacillus spp. are a group of thermophilic, acidophilic, spore-forming bacteria, some of which cause spoilage in commercially pasteurized fruit and vegetable juice products including apple, orange, tomato and carrot juice. In this study, we characterized seven isolates of Alicyclobacillus (14-2, KF, A-Gala 2-1, C-Fuji 6, Gala 9-2, 1016, 18-1) and a reference strain A. acidiphilus DSM 145...

Ovarian cancer, as well as other cancers, is primarily caused by methylation at cytosines in CpG islands, but the current marker for ovarian cancer is low in sensitivity and failed in early-stage detection. Fourier transform infrared (FT-IR) spectroscopy is powerful in analysis of functional groups within molecules, and infrared microscopy illustrates the location of specific groups within sing...