conclusions the synergistic effect was found on the healing of infected wounds caused by s. aureus by using an mbp-1 and silver nanoparticles combination in a mouse model. results the mic and mbc of mbp-1 were found to be 0.6 and 0.7 mg/ml, respectively. mic and mbc of silver nanoparticles were determined to be 6.25 and 12.5 mg/l, respectively. mic and mbc of the silver nanoparticles and mbp-1 ...

Nurses have shown a weakness both in recognising different types of exudate and in choosing an appropriate dressing (Smith and Gibson, 2011). In order to support nurses in identifying the correct dressing choice, an exudate wound decision algorithm has been developed. The algorithm suggests the type of dressing required for bleeding and infected wounds is a calcium alginate dressing with a silv...

Antimicrobial peptides (AMPs), represent promising agents for new therapeutic approaches of infected wound treatment, on account of their antimicrobial and wound closure activities, and low potential for inducing resistance. However, therapeutic applications of these AMPs are limited by their toxicity and low stability in vivo. Previously, we reported that the 23 amino-acid designer peptide TP3...

The relationship between wound, host and bacteria is continuously changing as a result of local, environmental and systemic factors (Templeton, 2014). One of these factors may be the use of antibiotics. However, due to the emergence of many resistant bacteria, the routine use of topical and oral antibiotics is not justified for colonised or infected wounds and should only be considered for woun...

Emerging bacterial resistance renders many antibiotics ineffective, making alternative strategies of wound disinfection important. Here the authors report on a new, physical burn wound disinfection method: pulsed electric fields (PEFs). High voltage, short PEFs create nonthermal, permanent damage to cell membranes, possibly by irreversible electroporation. In medicine, PEF technology has recent...

INTRODUCTION We present a standardized Pseudomonas aeruginosa (PA) infected burn-wound model in mice for evaluating new antimicrobials and therapy strategies for PA infections and demonstrate the effect of the antibiotic imipenem in this model. METHODS A 6%-8% total body surface area, full-thickness, scald-burn wound was induced in anesthetized mice. Two study groups (PA-infected burn) were c...

Staphylococcus aureus is a major human pathogen interfering with host-cell functions. Impaired wound healing is often observed in S aureus–infected wounds, yet, the underlying mechanisms are poorly defined. Here, we identify the extracellular adherence protein (Eap) of S aureus to be responsible for impaired wound healing. In a mouse wound-healing model wound closure was inhibited in the presen...

Staphylococcus aureus is a major human pathogen interfering with host-cell functions. Impaired wound healing is often observed in S aureus-infected wounds, yet, the underlying mechanisms are poorly defined. Here, we identify the extracellular adherence protein (Eap) of S aureus to be responsible for impaired wound healing. In a mouse wound-healing model wound closure was inhibited in the presen...

INTRODUCATION Negative pressure wound therapy (NPWT) represents an alternative method to optimize conditions for wound healing. Delayed wound closure is a significant health problem, which is directly associated with pain and suffering from patient's aspect, as well with social and financial burden. PRESENTATION OF CASE We report a case of vacuum-assisted wound therapy with hypertonic solutio...