نتایج جستجو برای: indirect immunofluorescence

تعداد نتایج: 102331  

Journal: :Arthritis Research & Therapy 2008
Michael Mahler Jennifer T Ngo Johannes Schulte-Pelkum Tanja Luettich Marvin J Fritzler

INTRODUCTION Autoantibodies to the ribosomal P proteins represent a highly specific marker for the diagnosis of systemic lupus erythematosus, where they have been associated with certain clinical manifestations. Historically, autoantibodies against ribosomal P proteins have been detected by indirect immunofluorescence, immunodiffusion, immunoblot, and other immunoassays. More recently, enzyme-l...

2017
Sarah Lonowski Hayley Goldbach Vanessa Holland

ELISA: enzyme-linked immunosorbent assay IIF: indirect Immunofluorescence PNP: paraneoplastic pemphigus PV: pemphigus vulgaris INTRODUCTION Because of variability in clinical and histopathologic findings, the diagnosis of paraneoplastic pemphigus (PNP) frequently relies on the combination of direct immunofluorescence and indirect immunofluorescence (IIF). Here we present a unique case of PNP as...

Journal: :BioMed research international 2016
Amel Benammar Elgaaied Donato Cascio Salvatore Bruno Maria Cristina Ciaccio Marco Cipolla Alessandro Fauci Rossella Morgante Vincenzo Taormina Yousr Gorgi Raja Marrakchi Triki Melika Ben Ahmed Hechmi Louzir Sadok Yalaoui Sfar Imene Yassine Issaoui Ahmed Abidi Myriam Ammar Walid Bedhiafi Oussama Ben Fraj Rym Bouhaha Khouloud Hamdi Koudhi Soumaya Bilel Neili Gati Asma Mariano Lucchese Maria Catanzaro Vincenza Barbara Ignazio Brusca Maria Fregapane Gaetano Amato Giuseppe Friscia Trai Neila Souayeh Turkia Haouami Youssra Raja Rekik Hayet Bouokez Maria Vasile Simone Francesco Fauci Giuseppe Raso

Antinuclear antibodies (ANAs) are significant biomarkers in the diagnosis of autoimmune diseases in humans, done by mean of Indirect ImmunoFluorescence (IIF) method, and performed by analyzing patterns and fluorescence intensity. This paper introduces the AIDA Project (autoimmunity: diagnosis assisted by computer) developed in the framework of an Italy-Tunisia cross-border cooperation and its p...

Background and aims: Toxoplasma gondii is an obligate intracellular parasite which has the potential toinfect all warm-blooded animals. Sheep and cattle play a main role in the economy and their infectionto T. gondii, besides economic losses, can cause the infection of humans through the consumptionof raw meat and other products. In this study, we aimed to survey the prevalenc...

Journal: :Journal of clinical microbiology 1985
E M Swierkosz M Q Arens R R Schmidt T Armstrong

An indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (HSV). The indirect immunofluorescence assay (Electro-Nucleonics, Inc.) correctly identified 16 HSV type 2 (HSV-2) isolates, but failed to identify 4 of 14 HSV-1 isolates because of background fluorescence and instability of reagents. Forty-nine HSV-1 is...

Journal: :Applied and environmental microbiology 1984
T W Hejkal E M Smith C P Gerba

A seasonal distribution was observed for rotaviruses in sewage by using indirect immunofluorescence. Levels were low from May through September and generally higher during winter and spring. In contrast, no seasonal pattern was observed for total enteroviruses. Limitations of the indirect immunofluorescence assay and enzyme immunoassay for environmental samples are discussed.

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