Background : Hypotonic shock induces cytocidal effects through cell rupture, and cancer therapy based on this mechanism has been clinically administered to hepatocellular carcinoma patients. We herein investigated the effectiveness of hypotonic shock combined with the inhibition of regulatory volume decrease as cancer therapy for hepatocellular carcinoma. Methods : Morphological changes in huma...

background: morphological changes of the cells infected with rubella virus cannot be observed easily. estimation of the size of the cultured cells can be a valuable parameter in this condition. this study was conducted to find answers to the following questions: how much time after infection with rubella virus, the volume and surface area of the vero cells and their nuclei get started to change...

in this trial, the effect of different levels of echum amoneum (ea) (0.3, 30, 300 or 3000 µl) on in vitro ruminal fermentation characteristics and methane production was assessed. the parameters studied were in vitro gas production (ivgp), methane production, ammonia (nh3-n) concentration, in vitro organic matter degradability (ivomde), partitioning factor (pf) (the ratio of substrate truly deg...

This article describes plug-based microfluidic technology that enables rapid detection and drug susceptibility screening of bacteria in samples, including complex biological matrices, without pre-incubation. Unlike conventional bacterial culture and detection methods, which rely on incubation of a sample to increase the concentration of bacteria to detectable levels, this method confines indivi...

The present study was carried out to determine the sex of the fertilized white layer eggs with using morphological measurements. A total of 300 Super Nick White Layer (54 wks of age) eggs were incubated and sexed in the end of incubation period. Before incubation egg length, width and weight were measured. The egg volume and shape index were estimated with using these measurements for each egg....

objective(s): carotenoids which are naturally synthesized by fungi such as yeasts can act as an antioxidant which is closely related to their ability to decrease the risk of a variety of degenerative diseases. in recent years, the increase of demand for carotenoids obtained from natural sources has promoted major efforts to improve carotenoid production from biological sources such as pigmented...

The mechanism of adrenergic swelling and associated pH changes was investigated in avian (goose) and teleost (striped bass and rainbow trout) erythrocytes. The swelling of goose red cells was probably caused by Na/K/Cl~ cotransport and consecutive osmotic flow of water into the cell. Goose red cells swelled when exposed to isoproterenol in the presence of elevated extracellular K, but not at ph...

Standard methods for the methyl red (MR) test are not practical for routine use in clinical laboratories because of the necessarily prolonged incubation period. When read after overnight incubation, the usual MR test is often equivocal or falsely positive. The present study demonstrates the importance of standardizing the total volume of broth, the size of the vessel in which the cultures are i...