A study was carried out to determine the effect of enzyme concentration, temperature and incubation time of bromelain on nitrogen content (NC) and degree of hydrolysis (DH) of hydrolysate from cockle (Anadara granosa) meat wash water. Protein precipitation of cockle meat wash water was conducted at pH 4. The precipitate was then hydrolyzed using bromelain at concentrations of 0.5, 1.5 and 2.5 %...

We wanted to know if incubation time and temperature for many radioimmunoassay methods could be standardized, to decrease assay time and so improve efficiency. Percentage binding was determined for triiodothyronine, thyroxine, digoxin, digitoxin, testosterone, aldosterone, estradiol, and diphenylhydantoin when the reaction mixtures were incubated at 6-8, 22, or 37 degrees C for various periods ...

Background: Temperature is the most important factor controlling embryonic development. Hyperand hypothermic effects depend on the degree of temperature, duration of exposure and the stage of the development. From the middle of incubation period, embryo temperature is greater than air temperature of the incubator because of the increased metabolic activity. Thus elevated temperature especially ...

We incubated eggs of Elaphe taeniura at 22, 24, 27, 30 and 32°C to examine the effects of incubation temperature on hatching success, embryonic use of energy and hatchling morphology. Incubation temperature affected incubation length and most hatchling traits examined in this study. Incubation length increased nonlinearly as temperature decreased, with the mean incubation length being 101.7 d a...

Avian parents that physically incubate their eggs must balance demands of self-maintenance with providing the proper thermal environment for egg development. Low incubation temperatures can lengthen the incubation period and produce changes in neonate phenotype that may influence subsequent survival and reproduction. We artificially incubated wood duck (Aix sponsa) eggs at three temperature reg...

Incubation temperature influences hatchling phenotypes such as sex, size, shape, color, behavior, and locomotor performance in many reptiles, and there is growing concern that global warming might adversely affect reptile populations by altering frequencies of hatchling phenotypes. Here I overview a recent theoretical model used to predict hatchling sex of reptiles with temperature-dependent se...

Frank, Hilmer A. (Eastern Regional Research Laboratory, Philadelphia, Pa.). Influence of low incubation temperature on classification of Pseudomonas geniculata. J. Bacteriol. 84:68-71. 1962.-Biochemical and physiological properties of psychrophilic Pseudomonas geniculata isolates were determined at 8 C and at 27 C. No qualitative differences in taxonomically significant properties were observed...

To investigate whether supplementing manganese (Mn) to the maternal diet could reduce the deleterious effect of heat stress on the developing embryo, the hatchability, antioxidant status, and expression of heat shock proteins (HSP) were evaluated in chick embryos under normal and high incubation temperatures. A completely randomized design ( = 6) with 2 maternal dietary Mn treatments (unsupplem...