Multimodal nanoprobes have attracted intensive attentions since they can integrate various imaging modalities to obtain complementary merits of single modality. Meanwhile, recent interest in laser-induced photoacoustic imaging is rapidly growing due to its unique advantages in visualizing tissue structure and function with high spatial resolution and satisfactory imaging depth. In this review, ...

Near-infrared fluorescent proteins, iRFPs, are recently developed genetically encoded fluorescent probes for deep-tissue in vivo imaging. Their functions depend on the corresponding fluorescence efficiencies and electronic excited state properties. Here we report the electronic excited state deactivation dynamics of the most red-shifted iRFPs: iRFP702, iRFP713 and iRFP720. Complementary measure...

The lack of efficient detection techniques has so far prevented ultrasound-modulated optical tomography from achieving maturity. By applying a quantum spectral filter based on spectral-hole burning, one modulation sideband of the ultrasound-modulated diffuse photons can be efficiently selected while the DC and the other sidebands are blocked. This technique features a large etendue as well as t...

Inorganic nanostructures that interface with biological systems have recently attracted widespread interest in biology and medicine. Nanoparticles are thought to have potential as novel intravascular probes for both diagnostic (e.g., imaging) and therapeutic purposes (e.g., drug delivery). Critical issues for successful nanoparticle delivery include the ability to target specific tissues and ce...

We describe a technique that uses biocompatible chemical agents to enhance both the sensitivity and the resolution of in vivo imaging with low-level light-emitting probes. We demonstrate experimentally, with chemiluminescence (CL) imaging in vitro as an example, that the detected intensity of CL from treated 3-mm-thick skin tissue is approximately fivefold stronger than that from untreated skin...

We show that phase-conjugation by three-wave mixing allows turbidity suppression through biological tissues with thicknesses up to 5 mm, at a near-infrared wavelength included in the therapeutic window. Because of the ultrafast character of the imaging process, a motion of the tissue, which mimics in vivo imaging, can be exploited to significantly improve the signal-to-noise ratio and the resol...

To gain insight into the dendritic structural changes that occur within minutes of ischemia, as well as circuit-level plasticity that occurs over days to weeks, it is necessary to devise stroke models for anesthetized transgenic mice that are compatible with in vivo imaging methods. In particular, we are interested in examining events during the first few minutes of ischemia that are associated...

Two mitochondria-targeted real-time probes were presented, which could selectively respond to hypochlorite over other ROS. Meanwhile, the "off-on" probes could be successfully applied in the in vivo imaging of hypochlorite in living mice.

Among the various anions, only cyanide induced the revival of fluoresecence of -Cu(2+) resulting in "Off-On" type sensing of cyanide, which can be monitored at pH 7.4 in 100% aqueous system, and has been applied to a microfluidic platform, in which fluorescent sensor -Cu(2+) displayed green fluorescence upon the addition of cyanide, the in vivo imaging of cyanide using Caenorhabditis elegans.