materials and methods the subjects included 27 male and 3 female patients with advanced chronic periodontitis. the mean age of the patients was 47.6 ± 2.0 years old. p. gingivalis was isolated from the samples and identified by various diagnostic tests, including gram staining, indol test, and fluorescent test. minimum inhibitory concentration (mic) of the essential oil against isolated p. ging...

background: vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. the objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. methods: oocytes were either cryopreserved as immature cumulus compact (imcc) (n=98 exp 1; ...

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

results the results obtained for each drug were compared with other drugs and also with the results of the control groups. the results related to promastigote and amastigote assays showed that when the dose of both drugs increased, the parasite number is reduced in comparison with the control groups. moreover, the parasitic burden in the test cultures decreased significantly. macrophage assay r...

objective: to evaluate the efficacy of in vitro sperm activation (isa) using non-liquefied versus liquefied asthenozoospermic semen samples for improvement of sperm parameters. materials and methods: fifty six oligoasthenozoospermic (oa) patients (age range: 22-44 years; mean: 32.089 years) were enrolled in this study. oa patients were classified according to type of infertility. also, duration...

traumatic brain injury (tbi) is caused by rapid deformation of the brain, resulting in a cascade of pathological events and ultimately neurodegeneration. in vitro models of tbi can help us to describe the pathobiological mechanisms. in vitro models of brain injury support a platform for performing repeatable, well-controlled, environmentally isolated experiments. other advantages of in vitro mo...