The effect of phosphorus supplementation to ammoniated rice straw was studied. The in vitro experiment was carried out following the first stage of Tilley and Terry method. The treatments consisting of four diets were A= 50% ammoniated rice straw + 50% concentrate (control), B= A + 0.2% phosphorus (P) supplement, C= A + 0.4% phosphorus (P) supplement, and D= A + 0.6% phosphorus (P) supplement o...

This study investigates the effects of three branched-chain amino acids (BCAA; valine, leucine, and isoleucine) on the in vitro ruminal fermentation of wheat straw using batch cultures of mixed ruminal microorganisms. BCAA were added to the buffered ruminal fluid at a concentration of 0, 2, 4, 7, or 10 mmol/L. After 72 h of anaerobic incubation, pH, volatile fatty acids (VFA), and ammonia nitro...

In order to provide recommendations on the most useful forage species to smallholder farmers, eleven grass and eleven legume forages grown in Abomey-Calavi in Republic of Benin were investigated for nutritive value (i.e. chemical composition and energy content) and fermentation characteristics (i.e. gas and volatile fatty acid production, organic matter degradability). The in vitro gas producti...

Awareness of the forage nutritive value of rangeland species is important to provide a balanced diet in order to meet the nutritional requirements of ruminants and to determine the suitable grazing time and rangeland grazing capacity. Hence, different laboratory methods were used to compare the nutritional value of Centaurea cyanus, Fumaria officinalis, Galium tricornutum and Vicia sativa as co...

Four in vitro experiments (Exp.) were conducted to evaluate lavender essential oil (LEO) effects at 0 (control), 250 (low dose), 500 (medium dose), 750 and 1000 µL per L(high doses) of incubation medium on rumen gas production kinetics (Exp.1), ruminal digestibility and fermentation (Exp.2), methane production (Exp.3) and rumen acidosis (Exp.4). The asymptote of gas production (A) incr...

Dry matter (DM) degradation of wheat straw (WS), barley straw (BS), lucerne hay (LH) and maize silage (MS) was determined using two different techniques: (i) in vitro gas production and (ii) nylon bag degradability technique. In vitro gas production and in situ DM disappearance were measured after 3, 6, 12, 24, 48, 72 and 96 hours of incubation. In situ and in vitro DM degradation kinetics was ...