The enzyme-linked immunosorbent assay (ELISA) was applied for immunological diagnosis of human tularemia, using lipopolysaccharide from Francisella tularensis as antigen. Sera collected from patients, healthy individuals, and vaccinated volunteers were investigated for antibodies against F. tularensis by ELISA and tube agglutination. In ELISA all sera were titrated with a polyspecific anti-immu...

This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity reaction, and in vitro lymphocyte proliferation assay) do not clearly differentiate between noninf...

Neurocysticercosis diagnosis is based on a combination of clinical, epidemiological, radiological, and immunological findings. We describe a real-time PCR assay for the confirmation of neurocysticercosis diagnosis in cerebrospinal fluid. The assay, tested on samples from nine patients living in France and diagnosed with neurocysticercosis, had a detection rate of 83.3% and 100% specificity.

Cellular immunological assays are important tools for the monitoring of responses to T-cell-inducing vaccine candidates. As these bioassays are often technically complex and require considerable experience, careful technology transfer between laboratories is critical if high quality, reproducible data that allows comparison between sites, is to be generated. The aim of this study, funded by the...

objective(s): according to the occupationally risk of infection in staff workers who have direct contact with mycobacterium species, we investigated their immunological parameters and compared with healthy purified protein derivative (ppd) negative volunteers.   materials and methods : we investigated 20 ppd positive volunteers working at tuberculin unit of razi vaccine and serum research insti...

introduction: cupping therapy (hejamat) is one of the therapeutic methods used in traditional medicine that it is still common in many of the contemporary societies. in spite of the progress of science, we have less information on the traditional and alternative medicine including cupping.   with the study of mechanism of action of cupping, it will be more convincing for the traditional medicin...

There is widespread interest in the immunostimulatory effects of food-derived substances that are expected to contribute improving human health. Broccoli ( Brassica oleracea var. italica ) has been shown contain potent polysaccharides for developing food materials with immunomodulatory function. In this study, activity previously uncharacterized broccoli was assessed using a silkworm Bombyx mor...

Proposal to develop an Improved immunological assay employing primary IgG antibodies and secondary IgM antibodies labeled with quantum dots to amplify antigen detection. Corresponding authors:[email protected], [email protected], [email protected]