AbstractNumerous deep learning based methods have been developed for nuclei segmentation H &E images and achieved close to human performance. However, direct application of such another modality images, as Immunohistochemistry (IHC) may not achieve satisfactory Thus, we a Generative Adversarial Network (GAN) approach translate an IHC image while preserving location morphology then apply pre-tra...

Neurofibroma of the adrenal glands is rare, most common site presentation are head and neck region along nerves. With us computed tomography , magnetic resonance imaging an ultrasonography for detection adrenals masses increasing. On neurogenic tumours frequently appear as a distinct, smooth or lobulated mass. All varieties may exhibit calcification But confirmation lesion histopathological exa...

background and objectives: epstein barr virus (ebv) is one of the members of herpesviridae family and a sub-category of gamma herpes virinae. ebv, which normally has cr2 or cd21 receptors on b-lymphocytes, has mutagenic features for them. the virus plays an important role in causing some malignant cancers. about 30% of the cases with non-hodgkin’s lymphoma are diffuse large b-cell lymphoma (dlb...

Immunohistochemistry (IHC) plays an essential role in Pathology. In order to improve reproducibility and standardization of the results interpretation, IHC quantification methods have been developed. IHC interpretation based in whole slide imaging or virtual microscopy is of special interest. The objective of this work is to review the different computer-based programs for automatic immunohisto...

Objectives Immunohistochemistry (IHC) is considered as a screening method for ALK rearrangement thanks to its excellent sensitivity. Strong marking on immunohistochemistry give the go-ahead start tyrosine kinase inhibitors (ALK TKI). Lack of therapeutic response may then lead suspicion molecular alterations other than rearrangements. Methods We present patient with strong and PD-L1 positive IHC...

The immunohistochemistry (IHC) with anti-BRAF V600E (VE1) mouse monoclonal antibody and DNA sequencing for BRAF V600E mutation was performed on 91 MSI-H colorectal specimens from patients tested for Lynch syndrome. Out of 91 cases 11 cases were positive for BRAF V600E mutation by Sanger sequencing and also by IHC. Seventy nine cases out of the remaining 80 cases classified as BRAF wild type sho...

The aim of this study was to develop and to optimize an immunohistochemistry (IHC) method for PCV2 identification and to compare it with an in situ hybridization (ISH) technique. The results demonstrated that both ISH and IHC successfully detected PCV2 viral antigens or nucleic acid in the examined tissues. Most of the slides identified previously in ISH as PCV2-positive were also positive in I...

Immunohistochemistry (IHC) plays an important role in biomarker-driven cancer therapy. Although there has been a high demand for standardized and quality assured IHC, it has rarely been achieved due to the complexity of IHC testing and the subjective validation-based process flow of IHC quality control. We present here a microfluidic immunostaining system for the standardization of IHC by creat...

BACKGROUND The advantage of tissue microarray (TMA) is its ability to efficiently analyze large numbers of tissue specimens in a methodologically uniform way. The reliability of TMAs, especially with regard to clinicopathological characterizations, when compared to conventional immunohistochemistry (IHC) was evaluated. MATERIALS AND METHODS Seventy-two embedded tissue sections from lung cance...