Although the use of a fluorescent antibody (FA) staining technique to demonstrate N. gonorrhoeae in secretions from patients with gonorrhoea was first described as long ago as 1959 by Deacon, Peacock, Freeman, and Harris, it does not seem to have gained wide acceptance in routine diagnosis. In our laboratory direct and delayed FA methods were first assessed in 1963 (Fry and Wilkinson, 1964). In...

PURPOSE To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. METHODS Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde and 0.2% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4, at 4 degrees C. After washing with 0.1% Triton X...

We compared two methods for identification of Chlamydia trachomatis inclusions in McCoy cell monolayers: conventional iodine staining and immunofluorescence staining with monoclonal antibodies against the species-specific major outer membrane protein antigen of C. trachomatis. Among 878 urethral and cervical specimens tested in parallel, the immunofluorescence method detected eightfold more inc...