A method for the isolation of rat 7S gamma globulin based on multiple adsorptions with DEAE-Sephadex A-50 has been presented. The separation of rat 7S globulin requires a procedure modified from the one used for humans in terms of the buffer pH, and the number of adsorptions. Ultracentrifugal analysis indicates the resulting fraction is a single component. In addition, immunoelectrophoresis of ...

The expression of human epidermal growth factor receptor 2 (HER2) on the surface of individual exosomes secreted from human breast cancer cells was evaluated by on-chip microcapillary immunoelectrophoresis. A positive shift of the zeta potential was observed for exosomes secreted from HER2positive SK-BR-3 cells, whereas no difference was observed for exosomes secreted from HER2-negative MDA-MB-...

Human platelet membranes were prepared by the glycerol-lysis technique of Barber and Jamieson (Barber, A. J., and Jamieson, G. A. (1970) J. Biol. Chem 245, 6357-6365), solubilized in 1% Triton X-100 and subjected to crossed immunoelectrophoresis, in 1% agarose, employing a rabbit anti-human platelet membrane antibody in the second dimension. Approximately 20 immunoprecipitates (numbered 1 to ...

Serum from a patient with multiple myeloma showed a monoclonal protein, classified by immunoelectrophoresis as IgD. Immunofixation electrophoresis and immunoelectrophoresis failed to demonstrate a precipitation reaction between the paraprotein and antisera to immunoglobulin light chains. The light chains of the monoclonal protein, immunologically inaccessible in the intact molecule, reacted wit...

The limits of detection of four classes of monoclonal immunoglobulin and free light chain in serum by isoelectric focusing and immunoisoelectric focusing have been determined and the sensitivity of these techniques compared with that obtained using immunoelectrophoresis and zonal electrophoresis with immunofixation. Immunoisoelectric focusing was 10-40 times more sensitive than immunoelectropho...

Analysis of Factor-VIII-related antigen (vWAg) in normal plasma by crossed immunoelectrophoresis reveals an asymmetric pattern indicative of molecular heterogeneity. This asymmetric pattern apparently results from the presence of multiple molecular forms of VWAg with distinct, though partially overlapping, mobilities on crossed immunoelectrophoresis. Evidence for this conclusion has been obtain...

SYNOPSIS The detection of antibacterial precipitins in the serum of patients with chronic lower respiratory infections has been shown to be of great value in the assessment of pathogenicity of the corresponding bacterial species in the sputum. The test used hitherto, however, is time-consuming and cumbersome owing to the need to distinguish, by immunoelectrophoresis, between antibodies specific...

To assess the validity of the multimeric analysis of von Willebrand factor (vWf), this technique was compared with two dimensional immunoelectrophoresis using samples of purified vWf obtained by gel filtration and plasma samples from a patient with severe von Willebrands disease who was receiving prophylaxis with cryoprecipitate. It is concluded that except for specialised clinical and research...