CONTEXT Immunoassay-based techniques, routinely used to measure serum estradiol (E2), are known to have reduced specificity, especially at lower concentrations, when compared with the gold standard technique of mass spectrometry (MS). Different measurement techniques may be responsible for the conflicting results of associations between serum E2 and clinical phenotypes in men. OBJECTIVE Our o...

Immunoassays represent one of the most popular analytical methods for detection and quantification of biomolecules. However, conventional immunoassays such as ELISA and flow cytometry, even though providing high sensitivity and specificity and multiplexing capability, can be labor-intensive and prone to human error, making them unsuitable for standardized clinical diagnoses. Using a commerciali...

The use of chemiluininescent molecules as non—isotopic alternatives to 125 in immunoassay permits the development of sensitive, stable assay systems.

We evaluated the performance of three commercial measles immunoglobulin M enzyme immunoassays from Meddens, Denka Seiken, and Behring. The sensitivities were determined to be 96.7% for the Meddens and Denka Seiken assays and 87.9% for the Behring assay. The specificities of the assays were determined to be 94.6% for Meddens, 98.2% for Denka Seiken, and 98.7% for Behring.

A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron mic...

We have modified the homogeneous enzyme immunoassay of thyroxine and have adapted it to the AutoChemist. Both enzyme immunoassay and radioimmunnoassay procedures were performed on 2016 patients' sera on which thyroxine determinations had been ordered. We find the automated enzyme immunoassay to be precise and accurate, and for the first time it permits total automation of an immunoassay on a mu...

This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reprodu...

BACKGROUND Immunoassay screening is used by pain physicians to determine compliance with controlled substances. Because clinical use of pain medications is different from illicit drug use, there is a need to evaluate the level of diagnostic accuracy of this procedure for the pain patient. OBJECTIVE To compare the results of automated screening by immunoassay with analysis by Liquid Chromatogr...

For point-of-care testing (POCT), a sandwich-typed immunoassay on poly(dimethylsiloxane) (PDMS) microfluidic chips was developed by employing two original technologies: a power-free microchip and laminar flow-assisted dendritic amplification (LFDA). Sequential injection of reagents of immunoassay was carried out by the power-free microchip. In addition, for the amplification of a fluorescent si...

Pesticides are used globally for enhancing crop yields. However, their excessive use/misuse, especially in the developing countries, results in widespread food and environmental contamination. The presence of pesticide residues in food and environment has posed a serious threat to human health and caused a great concern. In order to keep human from being affected, analytical and monitoring syst...