This paper is dedicated to the study on the external and internal mass transfers of glucose for succinic acid fermentation under substrate and product inhibitions using a bioreactor with stirred bed of immobilized Actinobacillus succinogenes cells. By means of the substrate mass balance for a single particle of biocatalysts, considering the kinetic model adapted for both inhibitory effects, spe...

Hybrid nano-biomaterials are exploited in the design and performance of chemo-enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) gold nanoparticles (Au NPs) on magnetic particles coated with silica ([email protected]2) to stepwise hydrolyze reduce p-nitrophenyl esters tandem reaction. The assembly two catalysts at interface [email protected]2 temp...

The immobilization of cells can be defined as ‘‘the physical confinement or localization of cells to a certain defined region of space with preservation of some desired activity’’ (1). The region in which the cells are localized is called the immobilized cell system or immobilized cells aggregate, which can be divided into three components: the cells, the support (or carrier or matrix) material...

Enzymes serve as biocatalysts for innumerable important reactions, however, their application has limitations, which can in many cases be overcome by using appropriate immobilization strategies. Here, a new support for immobilizing enzymes is proposed. This hybrid organic-inorganic support is composed of chitosan-a natural, nontoxic, biodegradable, and edible biopolymer-and sodium polyphosphate...

Immobilization of enzymes is one of the most promising methods in enzyme performance enhancement, including stability, recovery, and reusability. However, investigation of suitable solid support in enzyme immobilization is still a scientific challenge. Polyethersulfone (PES) and aminated PES (PES–NH2) were successfully synthesized as novel materials for immobilization. Membranes with various po...

Vinyl sulfone (VS)–agarose beads were used to develop a new strategy coimmobilize enzymes with very different stabilities, enabling the reuse of most stable immobilized enzyme. Two model combi-biocatalysts prepared. First, trypsin and chymotrypsin multipoint covalently coimmobilized on VS–agarose, support was blocked ethylenediamine, then β-galactosidase from Aspergillus oryzae via anion exchan...