The goal of this study was to measure arterial amino acid levels in patients with chronic heart failure (CHF), and relate them to left ventricular function and disease severity. Amino acids (AAs) play a crucial role for heart protein-energy metabolism. In heart failure, arterial AAs, which are the major determinant of AA uptake by the myocardium, are rarely measured. Forty-one subjects with cli...

Aristolochic acids (AAs) are a mixture of structural-related compounds, in which aristolochic acid I (AA I) and aristolochic acid II (AA II) are reported to be correlated with Aristolochic acid nephropathy (AAN). In this work, a rapid and sensitive ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to determine AA I and AA II in herbal products...

Preimplantation mouse embryos exhibit growth at the blastocyst stage when there is a dramatic increase in protein content between days 4 and 5 after hCG treatment. This increse in protein synthesis requires the uptake of amino acids from the surrounding medium, but the consumption of individual amino acids present as a mixture has not been examined. We therefore measured the simultaneous deplet...

Comparison of amino acid sequence similarity is the fundamental concept behind the protein phylogenetic tree formation. By virtue of this method, we can explain the evolutionary relationships, but further explanations are not possible unless sequences are studied through the chemical nature of individual amino acids. Here we develop a new methodology to characterize the protein sequences on the...

Some strains of the coagulase-negative Staphylococcus lugdunensis produce a synergistic hemolytic activity (SLUSH), phenotypically similar to the delta-hemolysin of S. aureus. Reverse-phase high-pressure liquid chromatography of supernatants from S. lugdunensis 307 yielded three late-eluting peaks of 3.5 kDa with synergistic hemolytic activity. A degenerate oligonucleotide probe was designed fr...

Aiming to develop a sustainable methodology for must acidity correction in winemaking, particularly needed warm regions, the present study intends fulfill circular economy values. Antão Vaz white wines were produced using two different strategies correction: (i) addition of mixture organic acids (Mix*) commonly used winemaking; and (ii) previously unripe grape (UM*) from same variety. In additi...

The ancient essential process of ribosomal protein synthesis requires twenty sets of aminoacyl-tRNAs (aa-tRNAs), one for each canonical amino acid. Since Crick proposed his adaptor hypothesis (1) it was commonly accepted that all organisms possess twenty aaRSs, each enzyme specific for attaching one amino acid to tRNA. It is now clear that aa-tRNA formation is more varied, as the biosynthetic r...

The Cobb × Cobb 500 slow-feathering male is a fast early-growth broiler grown under variable feeding programs and amino acid (AA) densities. An experiment was conducted using diets with increased ideal protein densities in a feeding program of 1 to 7 d, 8 to 21 d, 22 to 35d, and 36 to 42 d. Diets had ratios between essential digestible (D) AA and DLys (DAA:DLys) as follows: TSAA, 75%; DThr, 65%...