Abstract: This study was conducted to characterize infectious bursal disease virus (IBDV) isolates collected from different parts of Iran during 2005-2006. Pooled bursal samples from 49 broiler and layer pullet flocks suspected to IBD infection were collected and processed. A reverse transcriptase-polymerase chain reaction (RT PCR) procedure was used to amplify a VP2 gene fragment (743 bp) from...

The polyprotein of infectious bursal disease virus (IBDV), an avian birnavirus, is processed by the viral protease, VP4. Previous data obtained on the VP4 of infectious pancreatic necrosis virus (IPNV), a fish birnavirus, and comparative sequence analysis between IBDV and IPNV suggest that VP4 is an unusual eukaryotic serine protease that shares properties with prokaryotic leader peptidases and...

Infectious bursal disease virus (IBDV) isolates were recovered from outbreaks to initiate activities towards developing a local vaccine strain. Use of indigenous chicken embryos was exploited to determine their potential, promote utilization of local resources for research, and enhance household economic activities. Bursa of Fabricius (BFs) samples from outbreaks shown to be IBDV positive was h...

A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Beside...

Five weeks old guinea fowls were inoculated intraocularly with a 20% bursal suspension containing a local Nigerian isolate of infectious burfa! disease virus (IBDV) which had a bursal lesion titre of 10· per 0.5 mI. No clinical signs were observed. Gross lesions were absent and microscopic lesions were not found in the bursa, spleen and kidney on days 3 and 5 post infection (PI). IBDV antigen w...

The study was conducted to determine the prevalence of infectious bursal disease virus (IBDV) at different ages in commercial boilers. From Oct 2003 to Nov 2004, bursa samples collected from both vaccinated as well non-vaccinated commercially reared broiler chickens suspected of having infectious bursal disease were analyzed for the presence of IBDV using reverse transcriptase polymerase chain ...

Autophagy functions as an intrinsic antiviral defense. However, some viruses can subvert or even enhance host autophagic machinery to increase viral replication and pathogenesis. The role of autophagy during avibirnavirus infection, especially late stage infection, remains unclear. In this study, infectious bursal disease virus (IBDV) was used to investigate the role of autophagy in avibirnavir...

Infectious proventriculitis syndrome is at times a serious and ongoing problem causing significant financial losses. From the obtained results in the present study, it could be concluded that proventriculitis can be transmitted by oral inoculation of homogenates produced from proventriculi of birds with proventriculitis. Experimentally inoculated broilers were seroconverted to ARV, IBV and IBDV...

Infectious bursal disease virus (IBDV) infects young chickens and causes serious lose to the poultry industry, worldwide. Previous attempts using purified bacterially expressed IBDV VP2 failed to elicit a protective immune response to the virus. This study was designed to investigate if the initial expressed protein contained neutralizing epitopes but became nonfunctional during purification st...

necropsy, the proventriculi are enlarged with thickened walls. Microscopically, the glands are dilated, with necrosis of secretory cells, hyperplasia of ductular epithelium and mucosal and glandular inflammation caused by lymphocytic infiltration. The mucosa has cystic lesions and expansion of the lamina propria. These microscopic inflammatory lesions are distinct from lesions caused by noninfe...