We have studied inhibition of homogeneous human converting enzyme by a new inhibitor, a ketomethylene derivative of the blocked tripeptide substrate, Bz-Phe-Gly-Pro (ketoACE). KetoACE inhibited the hydrolysis of Hip-His-Leu and Hip-Phe-Arg at different concentrations (I50 values were 4 X 10(-8) M and 2 X 10(-7) M, respectively). Kinetic studies indicated that ketoACE inhibits the hydrolysis of ...

Organophosphate insecticides, such as Vapona, Naled, and Rabon, are highly potent inhibitors of an enzyme found in human monocytes. The enzyme, a specific monocyte esterase, could be inhibited by Vapona in blood samples via airborne contamination at levels easily achieved from commercial slow-release insecticide strips. Fifty percent inhibition (I50)--as measured on the Hemalog D (Technicon Cor...

A number of application for enzymes in organic solvents have been developed in chemical processing, food related conversions and analyses. The only unsolved problem related to nonaqueous enzymology is the notion that enzymes in organic solvent are mostly far less active than in water. Therefore, studies concerning the mechanisms by which enzymes are inactivated by organic solvents would reveal ...

This study aimed to determine the effects of compost amendment on soil properties, as well morphophysiological responses, seed yield, oil content, and fatty-acid profile. Nigella sativa plants under drought stress conditions. In a split-plot design, field experiment was carried out during two seasons (2020/2021 2021/2022), involving three irrigation regimes (named I100, I75, I50 crop evapotrans...

Sound irrigation and nitrogen management strategies are necessary to achieve sustainable yield water use efficiency of winter wheat in the North China Plain (NCP). The coupled effects scheduling application mode (NAM) on growth, under drip were evaluated with a two-year field experiment, which consisted three levels (ISLs) (irrigating when soil consumption (SWC) reached 20, 35 50 mm, referred a...

The inverse amplitude method is used to unitarize the two-loop pp scattering amplitudes of SU~2! chiral perturbation theory in the I50,J50,I51,J51, and I52,J50 channels. An error analysis in terms of the low energy one-loop parameters l̄ 1,2,3,4 and existing experimental data is undertaken. A comparison to standard resonance saturation values for the two-loop coefficients b̄1,2,3,4,5,6 is also ca...

Aim . This study aims to assess the incidence of heart failure (HF) and associated mortality, also burden on healthcare system in subjects Russian Federation, based HF encoding data. Material methods We made a structured request for number patients with cases providing medical care 2019. was understood mean presence at least one codes I09.9, I11.0, I13.0, I13.2, I25.5, I42.0, I42.5, I42.6, I42....

The pupil mechanism in the acone apposition eye of the semi-aquatic common backswimmer Notonecta glauca (Hemiptera) was investigated with infrared reflectometry of the pseudopupil. This method allows non-invasive continuous measurements of pupil responses in the living animal. The dynamic range of the pupil sensitivity is about 7 log units during daytime and 6 log units at night. During the day...

The present study focuses on the interaction between cadmium (Cd) and the Na, K-ATPase system in in vitro grown vascular smooth muscle cells (VSMCs) derived from the rat carotid artery. In disrupted VSMCs rendered permeable by osmotic shock, Cd inhibited Na, K-ATPase; I50 was reached at 10(-5) M Cd. Mg-ATPase was also inhibited by Cd; I50 was attained at concentrations of 10(-4) M Cd. Cd inhibi...

p-Hydroxyphenylpyruvate dioxygenase (HPPD) is a key enzyme in tyrosine catabolism and is the molecular target site of beta-triketone pharmacophores used to treat hypertyrosinemia in humans. In plants, HPPD is involved in the biosynthesis of prenyl quinones and tocopherols, and is the target site of beta-triketone herbicides. The beta-triketone-rich essential oil of manuka (Leptospermum scopariu...