The binding characteristics of corticotropin-releasing factor (CRF) type 1 (CRF(1)) and type 2 (CRF(2)) receptors from human (hCRF(1) and hCRF(2alpha)) and Xenopus (xCRF(1) and xCRF(2)) were compared using four different (125)I-labeled CRF analogs, the agonists (125)I-CRF and (125)I-sauvagine, and the antagonists (125)I-astressin ((125)I-AST) and (125)I-antisauvagine-30 ((125)I-aSVG). The hCRF(...

We describe a fast, PC-based optimization planning system for a planar permanent-seed implant. Sites where this system is applicable include brain, lung, and head and neck. The system described here allows placing ribbons of different strengths and of different lengths along and across the implant plane. The program takes full advantage of the availability of different source strengths in inven...

PURPOSE To present experimental dosimetry results for the new IsoSeed I25.S17plus (125)I brachytherapy source, in fulfillment of the American Association of Physicists in Medicine recommendation for, at least one, experimental dosimetry characterization of new low-energy seeds before their clinical implementation. METHODS AND MATERIALS A batch of 100 LiF thermoluminescent dosimeter (TLD)-100 ...

Radioimmunoassay (RIA) employing iodinated ligands represents a popular measurement method for small molecules due to its excellent sensitivity and specificity. Yet performing RIAs of large numbers of tubes remains a tedious laboratory chore due to the need to individually handle tubes multiple times. We here present a method in which conventional 125I-labeled RIA ([125I] RIA) is adapted to a m...

Background: Iodine brachytherapy sources with low photon energies have been widely used in treating cancerous tumors. Dosimetric parameters of brachytherapy sources should be determined according to AAPM TG-43U1 recommendations before clinical use. Monte Carlo codes are reliable tools in calculation of these parameters for brachytherapy sources. Materials and Methods: Dosimetric param...

We have previously used immunohistochemistry to show that the brain of the hagfish, Myxine glutinosa, contains a rich distribution of natriuretic peptide-immunoreactive elements with the densest distribution occurring in the telencephalon and the diencephalon. In this study, the distribution of (125)I-rat ANP and (125)I-porcine CNP binding sites was determined in the brain of M. glutinosa. The ...

This study employs (125)I-labeled phytochrome ((125)I-P) from oats to quantitate the binding of phytochrome to a membrane fraction from oats that is highly enriched for mitochondria, and it examines several parameters that influence this attachment. The binding of (125)I-Pfr to the mitochondrial fraction of unirradiated oat seedlings is significantly higher than that of (125)I-Pr. However, (125...