According to the producers of the lactobacillus vaccine, Solco Trichovac, its therapeutic effect in trichomoniasis is achieved by antibodies that are induced by the vaccination and cross react with Trichomonas vaginalis. Common antigens of Lactobacillus acidophilus from Solco Trichovac vaccine and T vaginalis were therefore sought by three different seroreactions. Immune serum against Lacidophi...

Hyperimmune rabbit antiserum to an early, nonstructural herpes simplex virus type 2 (HSV-2)-induced polypeptide (VP143) reacted in immunofluorescence tests with a variety of cell lines transformed by HSV-2. Cytoplasmic fluorescence was observed in 10 to 50% of HSV-2-transformed cells, whereas no fluorescence was observed in cells transformed by other oncogenic DNA viruses or by a chemical carci...

de Araujo, Wilson C. (National Institute of Dental Research, Bethesda, Md.), Eileen Varah, and Stephan E. Mergenhagen. Immunochemical analysis of human oral strains of Fusobacterium and Leptotrichia. J. Bacteriol. 86:837-844. 1963.-Lipopolysaccharides, isolated by phenol-water extraction of 27 strains of oral gram-negative bacteria conforming either to Fusobacterium polymorphum or Leptotrichia ...

The serological activities of the specific phenolic glycolipid I from Mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-M. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. High anti-phenolic glycolipid I immunoglobulin M antibodies wer...

We describe two enzyme-linked immunosorbent assays for rotavirus antigen in feces, which were designed to be as sensitive and specific as possible, and easy to use anywhere. Both are indirect methods, using the antibody capture method, but the second assay utilizes a rotavirus group-specific monoclonal "detecting" antibody instead of the hyperimmune polyvalent guinea pig antisera used in the fi...

Transmission experiments have shown that epidemic diarrhea of infant mice of the CFW strain is infectious and communicable. Cage to cage spread can take place by the air-borne route. The agent appears to be a fairly heat-resistant virus that can be serially transferred and that is neutralized by specific hyperimmune antiserum from rabbits. Mice are susceptible to it by the oral route from at le...

A biotinylated 3ABC recombinant protein was developed and used in a competitive ELISA (cELISA) to detect foot-and-mouth disease virus (FMDV) antibodies in cattle, sheep and pigs. In this report, we describe the cloning and expression of 3ABC protein in Escherichia coli cells as fusion protein with 6xHis and biotin. This cELISA uses streptavidin to capture bacterially expressed and in vivo bioti...

In previous studies with hyperimmune rabbit antisera, we found evidence of serologic cross-reactivity among Mycoplasma pneumoniae, Mycoplasma genitalium, and Mycoplasma gallisepticum. Because of certain biologic and morphologic similarities of these species, attempts were made to determine if this cross-reactivity related to the attachment protein (P1) of M. pneumoniae. Monoclonal and monospeci...

Human immune serum globulin (HISG) therapy is the mainstay of treatment for patients with antibody immunodeficiency. Treatment with ‘y-globulin allows many of these patients to remain symptomfree for prolonged periods. Three types of human immunoglobulin are available for therapeutic use: (1) regular HISG for intramuscular (IM) use, derived from a panel of normal donors, therefore containing a ...

K. Yamamoto reviewed the many different procedures which have been employed in attempting to eliminate mycoplasmas from contaminated cell cultures. These have included antibiotics (tetracyclines, kanamycin, chloramphenicol, novobiocin, tylosin, and gentamicin). In addition, surface-active compounds such as triton-X or SPS, gold salts, photodynamic inactivation, and hyperimmune sera have been ut...