نتایج جستجو برای: humanized

تعداد نتایج: 6443  

Journal: :Toxicological sciences : an official journal of the Society of Toxicology 2013
Prajakta P Albrecht Nicole E Torsell Prasad Krishnan David J Ehresman Steven R Frame Shu-Ching Chang John L Butenhoff Gerald L Kennedy Frank J Gonzalez Jeffrey M Peters

This study examined the effect of prenatal perfluorooctanoic acid (PFOA) administration on pre- and postnatal development using peroxisome proliferator-activated receptor α (PPARα)-humanized mice to determine if species differences in receptor activity might influence the developmental effects induced by PFOA. Pregnant mice were treated daily with water or PFOA (3mg/kg) by po gavage from gestat...

Journal: :The Journal of general virology 2006
Ann R Hunt Shana Frederickson Christopher Hinkel Katherine S Bowdish John T Roehrig

A humanized monoclonal antibody (mAb) has been developed and its potential to protect from or cure a Venezuelan equine encephalomyelitis virus (VEEV) infection was evaluated. The VEEV-neutralizing, protective murine mAb 3B4C-4 was humanized using combinatorial antibody libraries and phage-display technology. Humanized VEEV-binding Fabs were evaluated for virus-neutralizing capacity, then select...

2014
Feifei Xiong Liliang Xia Jingfang Wang Biao Wu Dengyu Wang Longfang Yuan Yating Cheng Hongying Zhu Xiaoyan Che Qinghua Zhang Guoping Zhao Ying Wang

Highly pathogenic avian influenza (HPAI) H5N1 virus infection is still a potential threat to public health worldwide. While vaccines and antiviral drugs are currently under development, neutralizing antibodies could offer an alternative strategy to prevent and treat H5N1 virus infection. In the present study, we had developed a humanized antibody against H5N1 viruses from mouse-derived hybridom...

2018
Sandeep Kumar Vishwakarma Avinash Bardia Chandrakala Lakkireddy Raju Nagarapu Md Aejaz Habeeb Aleem Ahmed Khan

AIM To develop appropriate humanized three-dimensional ex-vivo model system for drug testing. METHODS Bioengineered humanized livers were developed in this study using human hepatic stem cells repopulation within the acellularized liver scaffolds which mimics with the natural organ anatomy and physiology. Six cytochrome P-450 probes were used to enable efficient identification of drug metabol...

Journal: :Blood 2011
Chozhavendan Rathinam William T Poueymirou Jose Rojas Andrew J Murphy David M Valenzuela George D Yancopoulos Anthony Rongvaux Elizabeth E Eynon Markus G Manz Richard A Flavell

Humanized mouse models are useful tools to understand pathophysiology and to develop therapies for human diseases. While significant progress has been made in generating immunocompromised mice with a human hematopoietic system, there are still several shortcomings, one of which is poor human myelopoiesis. Here, we report that human CSF-1 knockin mice show augmented frequencies and functions of ...

Journal: :The Journal of infectious diseases 2015
Janin Knop Frank Hanses Teresa Leist Nancie M Archin Stefan Buchholz Joachim Gläsner André Gessner Anja K Wege

BACKGROUND Staphylococcus aureus is a common pathogen among humans worldwide, with an increasing prevalence of multidrug resistance. The understanding of virulence factors inducing pathogenicity is still incomplete, and thus far the transfer of results from animal studies into successful clinical trials has been difficult. METHODS In this study, we established an S. aureus infection model in ...

2018
Wannisa Khamaikawin Saki Shimizu Masakazu Kamata Ruth Cortado Yujin Jung Jennifer Lam Jing Wen Patrick Kim Yiming Xie Sanggu Kim Hubert Arokium Angela P. Presson Irvin S.Y. Chen Dong Sung An

Investigations of anti-HIV-1 human hematopoietic stem/progenitor cell (HSPC)-based gene therapy have been performed by HIV-1 challenge after the engraftment of gene-modified HSPCs in humanized mouse models. However, the clinical application of gene therapy is to treat HIV-1-infected patients. Here, we developed a new method to investigate an anti-HIV-1 HSPC-based gene therapy in humanized mice ...

Journal: :Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2013
Haifeng Zhang Liping Song Hongtu Ye Lide Hu Wenlu Liang Datao Liu

BACKGROUND Rituximab, a mouse Fab and human Fc chimeric antibody, has been widely used to treat Non-Hodgkin's lymphoma (NHL). However, only 48% of patients respond to the treatment and complete response rate is below 10%. Also, immunogenicity was reported in 17-20% patients receiving the treatment, making it unsuitable for long term diseases such as autoimmune disorders. It has been a hot resea...

2018
Emiko Tanida-Miyake Masato Koike Yasuo Uchiyama Isei Tanida

Green fluorescent protein (GFP) is tremendously useful for investigating many cellular and intracellular events. The monomeric GFP mNeonGreen is about 3- to 5-times brighter than GFP and monomeric enhanced GFP and shows high photostability. The maturation half-time of mNeonGreen is about 3-fold faster than that of monomeric enhanced GFP. However, the cDNA sequence encoding mNeonGreen contains s...

2014
Laurent Salphati Xiaoyan Chu Liangfu Chen Bhagwat Prasad Shannon Dallas Raymond Evers Donna Mamaril-Fishman Ethan G. Geier Jonathan Kehler Jeevan Kunta Mario Mezler Loic Laplanche Jodie Pang Anja Rode Matthew G. Soars Jashvant D. Unadkat Robert A. B. van Waterschoot Jocelyn Yabut Alfred H. Schinkel Nico Scheer

Organic anion transporting polypeptide (Oatp) 1a/1b knockout and OATP1B1 and -1B3 humanized mouse models are promising tools for studying the roles of these transporters in drug disposition. Detailed characterization of these models will help to better understand their utility for predicting clinical outcomes. To advance this approach, we carried out a comprehensive analysis of these mouse line...

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