The ability to successfully derive human embryonic stem cells (hESC) lines from human embryos following in vitro fertilization (IVF) opened up a plethora of potential applications of this technique. These cell lines could have been successfully used to increase our understanding of human developmental biology, transplantation medicine and the emerging science of regenerative medicine. The main ...

introduction: embryonic stem (es) cells are pluripotent cells derived from the inner cell mass of blastocysts. these cells are appropriate for creation of animal models of human genetic diseases, the study of gene functions in vivo and differentiation into specific types as potential therapeutic agents for several diseases. we describe here production of transgenic chimeric mouse blastocysts by...

Background: It was reported in a literature that approximately one third of the 4-cell stage embryos did not exhibit a tetrahedral shape. Non-tetrahedral embryos showed a lower in vitro developmental potential than tetrahedral embryos. Recently vitrification technology has been widely employed for embryo cryopreservation. The objective of this study was to prove our hypothesis that vitrified - ...