In this paper, we observed the co-localization of UBIAD1 and Ds Red-ER (an ER marker) in bladder carcinoma cell line T24 and human embryonic kidney cell line HEK293T. UBIAD1 accumulated on the endoplasmic reticulum (ER) in both T24 and HEK293T cell lines. Site-directed mutagenesis defined a LAY motif, positioned on 71-73 amino acids of the UBIAD1 N-terminus, as the ER retention signal of UBIAD1...

The human embryonic stem cell line, KthES11, is derived from a normal healthy blastocyst donated for clinical research. inner mass (ICM) was isolated using mechanical dissection and plated on laminin fragments. Cell line derivation, its propagation storage were performed without feeders in an animal product-free environment according to current Good Manufacturing Practice (cGMP) standards. KthE...

introduction: different effects of low level laser irradiation (llli) on various cell types have already been demonstrated. however, its effects on embryonic stem cells have not yet been shown. the present study evaluates the morphological and immunocytochemical effects of llli on human embryonic stem cell (hesc) colonies. material and methods: equal-sized pieces of hesc line (royan h1) were ir...

Epstein-Barr virus (EBV) infects human B lymphocytes and epithelial cells. We have compared the requirements for EBV glycoprotein-induced cell fusion between Chinese hamster ovary effecter cells and human B lymphoblasts or epithelial cells by using a virus-free cell fusion assay. EBV-encoded gB, gH, gL, and gp42 glycoproteins were required for efficient B cell fusion, whereas EBV gB, gH, and gL...

DOI: 10.1002/elps.202200149 The cover picture shows how capillary-channeled polymer (C-CP) fibers are applied in a spin-down tip format to isolate pure, concentrated populations of exosomes and other extracellular vesicles (EVs) from human embryonic kidney (HEK) cell culture media. Exosomes/EVs vehicles intercellular communication, containing genetic protein contents the mother cell. Beyond bei...

trying to use embryonic stem cells about 20 years ago, working with animals,  especially rats began. during these years , many experiments in mouse embryonic stem cells to transform into a variety of cells and transplanting them were led to remarkable success . in the next issue of human stem cells were considered successful until finally in 1998 the first report was published in the proliferat...

background and objective: pneumocystis pneumonia (pcp) has been historically the most prevalent opportunisticinfection in patients infected with the human immunodeficiencyvirus. culture of the organism has not been faced with suitable success in artificial media, while various results have been reported for cell culture media. the aim of this study was proliferation of pneumocystis carinii on t...

Cisplatin is one of the most useful chemotherapeutics which performs its cytotoxic effectvia accumulation of platinum resulting in oxidative stress, and destruction of cell DNA. Thiscould probably cause secondary cancers in healthy tissues. Lipocalin2 (Lcn2) is a protein whichits expression is increased in oxidative stresses. Therefore, the present study was performedto evaluate the protective ...