Cytosolic and nuclear O-linked N-acetylglucosaminylation has been proposed to be involved in the nuclear transport of cytosolic proteins. We have isolated nuclear and cytosolic N-acetyl-d-glucosamine (GlcNAc)-specific lectins from adult rat liver by affinity chromatography on immobilized GlcNAc and identified these lectins, by a proteomic approach, as belonging to the heat-shock protein (HSP)-7...

We have for the first time characterized the heat shock response in mycobacteria both at the level of transcription, by RNA extraction, Northern (RNA) blotting, and hybridization with gene-specific probes for the Mycobacterium tuberculosis 65- and 71-kDa heat shock proteins (HSPs), and at the level of translation, by [35S]methionine labelling, sodium dodecyl sulfate-polyacrylamide gel electroph...

This study evaluated associations of heat shock proteins (HSP) and an oxidative stress protein, protein deglycase (DJ1), with beef quality tenderness. Samples from the longissimus thoracis (N = 99) were collected pre-rigor (day 0) after 14-d aging. Warner-Bratzler shear force (WBSF), myofibrillar fragmentation index (MFI), a trained sensory panel used to determine meat quality. Protein abundanc...

The nonobese diabetic (NOD) mouse, a model of spontaneous insulin-dependent diabetes mellitus (IDDM), fails to express surface MHC class II I-Eg7 molecules due to a deletion in the E alpha gene promoter. E alpha-transgenic NOD mice express the E alpha E beta g7 dimer and fail to develop either insulitis or IDDM. A number of hypotheses have been proposed to explain the mechanisms of protection, ...

Immunohistochemical demonstration of tumor-associated markers may be valuable as an adjunct to conventional histology for determination of malignancy development in oral mucosal diseases. Accordingly, the present study determined the distribution of some important tumor markers such as heat shock proteins (HSP), fibroblast growth factors (FGF) and transferrin receptors (TF-R) in 22 samples ...