نتایج جستجو برای: hplc method
تعداد نتایج: 1651320 فیلتر نتایج به سال:
In this study, quantitative and pattern recognition analyses were developed using HPLC/UV for the quality evaluation of Dipsaci Radix. For quantitative analysis, five major bioactive compounds were assessed. The separation conditions employed for HPLC/UV were optimized using ODS C18 column (250 × 4.6 mm, 5 μm) with a gradient of acetonitrile and water as the mobile phase at a flow rate of 1.0 m...
This work proposes a procedure to estimate the measurement uncertainty for the quantification of cefazolin by high-performance liquid chromatography (HPLC) and microbiological assay (bioassay). HPLC method was performed according to United States Pharmacopeia (USP) and bioassay according to Brazilian Pharmacopeia (FB). The quantification of cefazolin by high-performance liquid chromatography wa...
In this article, we describe a fast and specific method to measure 5FU with HPLC tandem-mass spectrometry. Reversed-phase HPLC was combined with electrospray ionization tandem mass spectrometry and detection was performed by multiple-reaction monitoring. Stable-isotope-labeled 5FU (1,3-15N2-5FU) was used as an internal standard. 5FU was measured within a single analytical run of 16 min with a l...
Black cohosh has been widely used as an herbal medicine for the treatment of symptoms related to menopause in America and Europe during the past several decades, but the bioactive constituents are still unknown. Formononetin is an isoflavone with known estrogen-like activity. This compound was first reported to be isolated from black cohosh in 1985, but subsequent research in 2002 using HPLC-PD...
As one of the widely used veterinary macrolide antibiotics, the environmental risks of tylosin (TYL) received more and more attention. The purpose of the present study was to develop a modified, rapid and precise RP-HPLC method with PDA detection for the determination of the macrolide antibiotic tylosin in liquid samples. The proposed two HPLC methods offered short retention time (< 6.8 min), h...
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