نتایج جستجو برای: host cell dna contamination
تعداد نتایج: 2228512 فیلتر نتایج به سال:
Background: Diazinon is one of the most common pesticides in the world playing a similar role to radiation and it could cause DNA breaks and genetic effects.Objective: In this study, radiosensitivity of a lymphoblastic cell line pretreated by Diazinon was investigated. Material and Methods: In this case-control study, the human lymphoblastic T-cell line was divided into 6 groups bas...
allogeneic hematopoietic stem cell transplantation (hsct) is a curative treatment option for hematological disorders. cyclosporine (csa) is one of the major immunosuppressive agents for the prophylaxis against graft versus host disease (gvhd). in this retrospective study, we evaluated the effects of csa serum levels on the incidence of acute gvhd and transplant outcomes. 103 adult patients rece...
Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign DNA across the cell membrane, and integration into host genomic DNA or maintenance of an autonomous vector DNA inside the host cell. This review introduces limitations and obstacles relat...
Methods to assess the impact of goose fecal contamination are needed as the result of the increasing number of Canada geese (Branta canadensis) near North American inland waters. However, there is little information on goose fecal microbial communities, and such data are important for the development of host-specific source-tracking methods. To address this issue, 16S rRNA gene clone libraries ...
Background & Objective: DNA vaccines as a new generation of vaccines require adjuvant to improve vaccine immunogenicity; adjuvants can also increase the DNA vaccine efficacy. In this study, the effects of the host’s interferon-inducible Mx protein as bio adjuvant and conventional alum adjuvant were evaluated. Materials & Methods: The BALB/c mice were immunized by different prime-boost strate...
BACKGROUND Autologous chondrocyte implantation (ACI) is a cell-based treatment that can be used to regenerate chondral defects. European legislation specifically classifies such produced chondrocytes as "medicinal for advanced cell therapy" that have to be manufactured in pharmaceutical factories according to specific rules, named Good Manufacturing Practices (GMPs). One main requirement of cel...
Research on ancient DNA (aDNA) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. However, several major problems in this field limit the applicability of aDNA studies, most importantly contamination with modern DNA and postmortem DNA degradation. In this study we extracted and analyzed aDNA obtained from ~3...
High-throughput sequencing technologies have strongly impacted microbiology, providing a rapid and cost-effective way of generating draft genomes and exploring microbial diversity. However, sequences obtained from impure nucleic acid preparations may contain DNA from sources other than the sample. Those sequence contaminations are a serious concern to the quality of the data used for downstream...
Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing properties of viral and vertebrate DNA. Shortly after infection of HeLa cells wit...
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