The bacterial flagellar hook acts as a universal joint to smoothly transmit torque produced by the motor to the filament. The hook protein FlgE assembles into a 55 nm tubular structure with the help of the hook cap (FlgD). FlgE consists of four domains, D0, Dc, D1 and D2, arranged from the inner to the outer part of the tubular structure of the hook. The Dc domain contributes to the structural ...

The formation of the apical hook in dicotyledonous seedlings is believed to be effected by gravity in the dark. However, this notion is mostly based on experiments with the hook formed on the hypocotyl, and no detailed studies are available with the developmental manners of the hook, particularly of the epicotyl hook. The present study aims at clarifying the dynamics of hook formation including...

The Drosophila hook gene encodes a novel component of the endocytic compartment. Previously identified hook alleles, which still expressed truncated Hook proteins, affected the accumulation of internalized transmembrane ligands into multivesicular bodies (MVBs). To determine the hook null phenotype, we isolated nine new hook alleles on the basis of their characteristic hooked-bristle phenotype....

introduction: one of the most common complications of root canal treatment is postoperative pain. the aim of the present clinical trial was to compare the severity of postoperative pain after root canal preparation with race rotary system and hand k-flexofile. methods and materials: a total of 96 mandibular first and second molars were divided into two groups ( n =48) based on root canal prepar...

The flagellar hook of Salmonella is a filamentous polymer made up of subunits of the protein FlgE. Hook assembly is terminated when the length reaches about 55 nm. After our recent study of the effect of cellular levels of the hook length control protein FliK, we have now analyzed the effect of cellular levels of FlgE itself. When FlgE was overproduced in a wild-type strain, a fliC (flagellin) ...

Arabidopsis seedlings develop a hook-like structure at the apical part of the hypocotyl when grown in darkness. Differential cell growth processes result in the curved hypocotyl hook. Time-dependent analyses of the hypocotyl showed that the apical hook is formed during an early phase of seedling growth and is maintained in a sequential phase by a distinct process. Based on developmental genetic...

Transmembrane ligands can be internalized across cell boundaries into receptor-expressing cells. In the developing Drosophila eye imaginal disc, the bride of sevenless transmembrane protein (boss) is expressed on the surface of R8 cells. After internalization into neighboring R7 cells, the boss protein accumulates in multivesicular bodies. In a search for genes that affect this cell-type-specif...

AT-rich DNA, and the proteins that bind it (AT-hook proteins), modulate chromosome structure and function in most eukaryotes. Unlike other trypanosomatids, the genome of Leishmania species is unusually GC-rich, and the regulation of Leishmania chromosome structure, replication, partitioning is not fully understood. Because AT-hook proteins modulate these functions in other eukaryotes, we examin...

An outstanding square slot element, for x-band wireless applications with asymmetric coplanar waveguide fed (CPW) is proposed. Various shapes of metallic strips are utilized as grounded stubs for providing Right Hand Circular Polarization (RHCP) functionality and wide-band properties. By adjusting the proposed stubs and hook-shaped radiator, length and positions, wider impedance band width is o...

Spinal surgery has evolved from early Egyptian times to the present through generations of new concepts, designs, procedures, and technology. In 1862, Edwin Smith discovered the works of Imhotep, Vizer of Djoser, who recorded his treatment of spinal cord injury, as well as spinal dislocation and subluxation, around 2000 B.C.11,20 In 1882, William F. Wilkins described and performed the first spi...