The DNA binding properties of three novel extended aromatic bisamidines (1-3) possessing different dicationic terminal side chains were studied. Data from the ethidium displacement assay showed that bisamidines 1-3 have significant affinity for DNA. We studied the cytotoxic activity of bisamidines 1-3 and Hoechst 33258 in the cultured breast cancer MCF-7 cells. These data show that in broad ter...

The denaturation behaviour of DNA-protein complexes in metaphase chromosomes in suspension was analysed in situ by Hoechst 33258 fluorescence. The results indicate that due to the stability of the dye molecule and the product of the molecular extinction coefficient and the quantum yield at different temperatures, Hoechst 33258 is a suitable probe for the detection of double-stranded DNA. Thus, ...

Free solution capillary electrophoresis (FSCE) has been used to separate two non-self-complementary 12mer oligonucleotide duplexes: d(AAATTATATTAT).d(ATAA-TATAATTT) and d(GGGCCGCGCCGC).d(GCGGCGCGGCCC). Titration of mixtures of the two oligonucleotides with model intercalators (ethidium bromide andactinomycin D) and minor groove binders (netropsin, Hoechst 33258 and distamycin) has shown the sui...

Molecular assemblies of potential guest molecules through non-covalent host-guest interactions have found immense use in many applied areas. In this study supramolecular interaction of a biologically important dye Hoechst-33258 (H33258) has been investigated in aqueous solutions at different pHs, in the presence of a macrocyclic host, namely, cucurbit[7]uril (CB7). The pH dependent emission beh...

We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of compact-globular and hollow-toroidal particles. In order to explore the functio...

The binding sites of Hoechst 33258, netropsin and distamycin on three DNA restriction fragments from plasmid pBR322 were compared by footprinting with methidiumpropyl-EDTA X Fe(II) [MPE X Fe(II)]. Hoechst, netropsin and distamycin share common binding sites that are five +/- one bp in size and rich in A X T DNA base pairs. The five base pair protection patterns for Hoechst may result from a cen...

the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

calprotectin, a calcium-bound protein complex, is abundant in the cytosol of neutrophils. it has been reported that this protein has an apoptotic activity in tumor cells. since calprotectin increases in colorectal cancer, this study was conducted to investigate, for the first time, the cytotoxicity/apoptotic effect of calprotectin on ht29/219 and sw742 colon carcinoma and adenocarcinoma cell li...

The DNA binding properties of three novel extended aromatic bisamidines (1–3) possessing different dicationic terminal side chains were studied. Data from the ethidium displacement assay showed that bisamidines 1–3 have significant affinity for DNA. We studied the cytotoxic activity of bisamidines 1–3 and Hoechst 33258 in the cultured breast cancer MCF-7 cells. These data show that in broad ter...