Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

The sonnet which is originated in Italy a highly structured poetic form. It flourishes the Elizabethan period order to write love sonnets about beloved and idealized lady. This study focuses on theme of time both Edmund Spenser’s Amoretti Epithalamion, William Shakespeare’s Sonnets. Amoretti, covers New Year’s Day celebrations, reflects past forty-one years life. In Spenser records hours day fr...

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

To my beloved families. iv ACKNOWLEDGEMENTS First, I would like to thank my thesis advisor, Dr. Elisa Riedo, for providing me the opportunity to conduct this exciting research. I'm sincerely grateful for her valuable guidance and perpetual encouragement though my entire graduate research. and his group, for the fabrication of thermal cantilevers. their great help and support. v Finally, I would...

The genre of the diary was widespread in Russian literature 1920s–1930s. most famous works are “Cursed Days” by I. Bunin, “Diary” L. Andreev. M. Prishvin’s “Diaries” among voluminous and significant literature. is especially vivid period from 1905 to 1954 with selected themes foreground: world nature, relations iconic personalities historical period, events author’s own life, which at same time...

The research material is Tropic of Cancer, a 1934 novel by the American novelist Henry Miller, and “Disintegration Atom”, 1938 story Russian poet Georgy Ivanov. aim article to compare plot situations self-identification emigrant heroes in terms manifestation their national identity order understand how problem selfidentification person modern era exile exacerbated. first draws attention stories...

A commercial medium often assumed to exist solely for a juvenile and "lowbrow" audience, comic books have been largely ignored by academic scholarship. There are some exceptions within the fields of American studies-for instance, the work of Thomas Inge-popular culture studies, where writers gather in a Comic Arts subdivision of the Popular Culture Association, and cultural studies, with work b...